Surface Free Energy Dominates the Biological Interactions of Postprocessed Additively Manufactured Ti-6Al-4V.
Surface Free Energy Dominates the Biological Interactions of Postprocessed Additively Manufactured Ti-6Al-4V.
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DOI：
10.1021/acsbiomaterials.2c00298
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发表时间：
2022-10-10
影响因子：
5.8
通讯作者：
Cox, Sophie C.
中科院分区：
文献类型：
--
作者：
Puzas, Victor Manuel Villapun;Carter, Luke N.;Schroder, Christian;Colavita, Paula E.;Hoey, David A.;Webber, Mark A.;Addison, Owen;Shepherd, Duncan E. T.;Attallah, Moataz M.;Grover, Liam M.;Cox, Sophie C.
关键词：
由于能够提供个性化的设备，加法制造(AM)已经成为医疗保健领域的一项颠覆性技术；然而，印刷金属部件仍然存在表面和微结构缺陷，这可能会危及机械和生物相互作用。这使得物理和/或化学后处理技术对金属AM设备至关重要，尽管关于物理化学性质的变化如何影响AM生物结果的基础知识有限。为此，本文对粉末床熔化Ti-6Al-4V样品进行了三种工业相关技术的后处理：抛光、钝化和振动抛光。从粗糙度、化学成分、润湿性、表面自由能和表面ζ势等方面对这些表面进行了全面的表征。在抛光和钝化样品上都观察到表皮葡萄球菌定植的显著增加，这与表面自由能供体γ-在酸碱γAB组分中的高值有关。早期成骨细胞附着和增殖(24小时)不受这些特性的影响，尽管这两个样本都观察到矿化增加。相比之下，不锈钢上的成骨细胞分化是由粗糙度和化学成分共同驱动的。总而言之，这项研究强调了表面自由能是AM表面和细胞相互作用之间的关键驱动因素。特别是，虽然低酸碱成分导致了表皮葡萄球菌定殖率的理想减少，但随后矿化作用也减少了。因此，虽然表面自由能可以用来指导AM设备的开发，但细菌和哺乳动物细胞相互作用的优化应该通过不同的后处理技术的组合来实现。
Additive manufacturing (AM) has emerged as a disruptive technique within healthcare because of its ability to provide personalized devices; however, printed metal parts still present surface and microstructural defects, which may compromise mechanical and biological interactions. This has made physical and/or chemical postprocessing techniques essential for metal AM devices, although limited fundamental knowledge is available on how alterations in physicochemical properties influence AM biological outcomes. For this purpose, herein, powder bed fusion Ti-6Al-4V samples were postprocessed with three industrially relevant techniques: polishing, passivation, and vibratory finishing. These surfaces were thoroughly characterized in terms of roughness, chemistry, wettability, surface free energy, and surface ζ-potential. A significant increase in Staphylococcus epidermidis colonization was observed on both polished and passivated samples, which was linked to high surface free energy donor γ– values in the acid–base, γAB component. Early osteoblast attachment and proliferation (24 h) were not influenced by these properties, although increased mineralization was observed for both these samples. In contrast, osteoblast differentiation on stainless steel was driven by a combination of roughness and chemistry. Collectively, this study highlights that surface free energy is a key driver between AM surfaces and cell interactions. In particular, while low acid–base components resulted in a desired reduction in S. epidermidis colonization, this was followed by reduced mineralization. Thus, while surface free energy can be used as a guide to AM device development, optimization of bacterial and mammalian cell interactions should be attained through a combination of different postprocessing techniques.
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影响因子：
8.2
作者：
Damiati L;Eales MG;Nobbs AH;Su B;Tsimbouri PM;Salmeron-Sanchez M;Dalby MJ
通讯作者：
Dalby MJ
DOI：
10.1186/s13046-020-01685-w
发表时间：
2020-09-04
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Cui J;Dean D;Hornicek FJ;Chen Z;Duan Z
通讯作者：
Duan Z
影响因子：
4.1
作者：
Fernandes, Russell J.;Harkey, Michael A.;Eyre, David R.
通讯作者：
Eyre, David R.
DOI：
10.1007/s10856-017-5972-x
发表时间：
2017-11-01
影响因子：
3.7
作者：
Echeverry-Rendon, Monica;Galvis, Oscar;Echeverria, Felix
通讯作者：
Echeverria, Felix
DOI：
10.1016/j.msec.2020.111631
发表时间：
2021-02-01
影响因子：
7.9
作者：
Bernhardt, Anne;Schneider, Jakob;Botzenhart, Ute
通讯作者：
Botzenhart, Ute