Changes in ATP, phosphocreatine, and 16 metabolites in muscle stimulated for up to 96 hours.
Changes in ATP, phosphocreatine, and 16 metabolites in muscle stimulated for up to 96 hours.
复制标题
刺激长达 96 小时的肌肉中 ATP、磷酸肌酸和 16 种代谢物的变化。
DOI：
10.1152/ajpcell.1996.271.4.c1167
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Lowry,OH
中科院分区：
文献类型：
--
作者：
Salmons,S;Jarvis,JC;Mayne,CN;Chi,MM;Manchester,JK;McDougalJr,DB;Lowry,OH
Rabbit tibialis anterior muscles were stimulated continuously at 10 Hz for periods ranging from 2 min to 96 h and were analyzed for energy reserves and metabolic intermediates. Glycogen, ATP and phosphocreatine fell rapidly during the first 5 min of stimulation. Glycogen continued to fall to very low levels, whereas ATP and phosphocreatine rose, reaching 70% of control by 1 h, despite ongoing stimulation. After 2 h, glycogen also increased, regaining control levels in 4 days. Glucose rose to 4.5 times control in 30 min and still exceeded 2.5 times control at 24 h. In the first 2 min, glycolytic intermediates, glucose 6-phosphate (G-6-P), fructose 1,6-bisphosphate, lactate, and pyruvate more than doubled and then returned to control levels or below. Malate and 3-glycerophosphate rose 600 and 200%, respectively. Both of these compounds participate in shuttling reducing equivalents from cytoplasm into mitochondria. Citrate and alpha-ketoglutarate underwent much more modest changes. Glucose 1,6-bisphosphate (G-1,6-P2) fell to one-third of control by 2 h and then rose dramatically at 4 h. At 4 days it was still twice control. The 6-phosphogluconate (6PG) doubled at 2 min, then rose to 12 times control at 2 h, fell somewhat, and peaked at 16 times control at 24 h. Aspartate and alanine both exhibited a biphasic rise in concentration, whereas glutamate fell to 30% in 15 min and rose slowly after 4 h. The rise in glucose was interpreted to be the consequence of rapid glycogenolysis together with inhibition of hexokinase by G-1,6-P2 and elevated G-6-P. Paradoxically, glycogen resynthesis apparently occurred when the glycogen synthase stimulator, G-6-P, was very low, and the glycolysis stimulator, G-1,6-P2, was high. Although G-1,6-P2 is an inhibitor of 6PG dehydrogenase, the timing of the changes in G-1,6-P2 and 6PG levels suggests that the accumulation of 6PG was initiated by some other influence.
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DOI：
10.1016/0005-2744(75)90125-4
发表时间：
1975-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BEITNER, R;HABERMAN, S;LIVNI, L
通讯作者：
LIVNI, L
影响因子：
13.8
作者：
BEITNER, R
通讯作者：
BEITNER, R
哺乳动物肌肉的慢性刺激：单个纤维中代谢物浓度的变化。
DOI：
10.1152/ajpcell.1988.255.4.c543
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
--
作者：
Henriksson,J;Salmons,S;Chi,MY;Hintz,CS;Lowry,OH
通讯作者：
Lowry,OH
己糖激酶葡萄糖-6-P 抑制位点的特异性。
影响因子：
3.9
作者：
I. A. Rose;J. V. Warms;D. Kosow
通讯作者：
D. Kosow
低频刺激兔快肌中葡萄糖 1,6-二磷酸和果糖 2,6-二磷酸水平的改变
影响因子：
3.5
作者：
H. Green;Juan Cadefau;D. Pette
通讯作者：
D. Pette