A paper-based multiplexed transaminase test for low-cost, point-of-care liver function testing.
A paper-based multiplexed transaminase test for low-cost, point-of-care liver function testing.
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DOI：
10.1126/scitranslmed.3003981
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发表时间：
2012-09-19
影响因子：
17.1
通讯作者：
Whitesides GM
中科院分区：
文献类型：
--
作者：
Pollock NR;Rolland JP;Kumar S;Beattie PD;Jain S;Noubary F;Wong VL;Pohlmann RA;Ryan US;Whitesides GM
In developed nations, monitoring for drug-induced liver injury via serial measurements of serum transaminases (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) in at-risk individuals is the standard of care. Despite the need, monitoring for drug-related hepatotoxicity in resource-limited settings is often limited by expense and logistics, even for patients at highest risk. This manuscript describes the development and clinical testing of a paper-based, multiplexed microfluidic assay designed for rapid, semi-quantitative measurement of AST and ALT in a fingerstick specimen. Using 223 clinical specimens obtained by venipuncture and 10 fingerstick specimens from healthy volunteers, we have shown that our assay can, in 15 minutes, provide visual measurements of AST and ALT in whole blood or serum which allow the user to place those values into one of three readout “bins” (<3x upper limit of normal (ULN), 3-5x ULN, and >5x ULN, corresponding to tuberculosis/HIV treatment guidelines) with >90% accuracy. These data suggest that the ultimate point-of-care fingerstick device will have high impact on patient care in low-resource settings.
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