Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress.
Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress.
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DOI：
10.1186/1472-6882-14-392
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发表时间：
2014-10-13
影响因子：
--
通讯作者：
Marnewick JL
中科院分区：
文献类型：
--
作者：
Ajuwon OR;Oguntibeju OO;Marnewick JL
腹腔注射脂多糖（LPS）后可引起急性肝损伤。氧化应激和促炎细胞因子的释放均参与LPS诱导的急性肝损伤的发病机制。本研究探讨了发酵的路易波士（Aspalathus linearis）提取物对LPS诱导的急性肝损伤的改善作用。在实验开始之前，通过HPLC-DAD对发酵的路易波士提取物中的主要酚类化合物以及体外抗氧化能力进行定量。将雄性Wistar大鼠随机分为4组（每组n = 10），并在注射LPS之前给予水或发酵的路易波士提取物4周。在大鼠中监测肝功能标志物，包括转氨酶和乳酸脱氢酶、脂质过氧化标志物、抗氧化酶、谷胱甘肽氧化还原状态以及细胞因子水平。注射LPS后，血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）水平显著升高。氧化应激，证明了增加的硫代巴比妥酸反应物质（TBARS）的血浆和肝脏中的丙二醛（MDA），全血和肝脏中的谷胱甘肽氧化还原状态（GSH：GSSG比）降低诱导LPS攻击的大鼠。此外，促炎反应标志物TNF-α、IL-1β和IL-6的肝脏水平显著升高。预先饲喂发酵的路易波士提取物4周降低了LPS诱导的血清AST和LDH（显著，p < 0.05）以及ALT的升高水平。食用路易波士引起的血浆和肝脏MDA的增加，全血和肝脏GSH：GSSG比例下降，以及各种抗氧化酶的变化观察到的衰减。TNF-α和IL-6的升高被显著抑制，表明路易波士抑制了诱导的炎症反应。总体而言，我们的数据表明，路易波士水提取物可能通过调节氧化应激和抑制促炎细胞因子的形成来减轻LPS诱导的肝损伤。
Acute liver injury occur after intraperitoneal administration of lipopolysaccharide (LPS). Oxidative stress and release of pro-inflammatory cytokines are both implicated in the pathogenesis of LPS-induced acute liver injury. This study investigated the ameliorative effect of fermented rooibos (Aspalathus linearis) extract on LPS-induced acute liver injury. Major phenolic compounds in the fermented rooibos extract by HPLC-DAD, as well as the in vitro antioxidant capacity were quantified before the start of the experiment. Male Wistar rats were randomized into 4 groups (n = 10 per group) and given either water or fermented rooibos extract for 4 weeks before LPS injection. Hepatic function markers, including aminotransferases and lactate dehydrogenase, lipid peroxidation markers, antioxidant enzymes, glutathione redox status, as well as cytokine levels were monitored in the rats. Injection of LPS significantly increased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH). Oxidative stress, evidenced by increased thiobarbituric acid reactive substances (TBARS) measured as malondialdehyde (MDA) in plasma and liver, and decreased glutathione redox status (GSH: GSSG ratio) in whole blood and liver was induced in LPS-challenged rats. Furthermore, hepatic levels of pro-inflammatory response markers TNF-α, IL-1β and IL-6 were increased significantly. Pre-feeding the fermented rooibos extract for 4 weeks decreased LPS-induced elevated levels of serum AST and LDH (significantly, p < 0.05) as well as ALT marginally. Consuming rooibos caused an attenuation of the observed increase in plasma and hepatic MDA, decrease in whole blood and liver GSH:GSSG ratio, as well as the changes noted in various antioxidant enzymes. The elevation in TNF-α and IL-6 was significantly suppressed, indicating an inhibition of the induced inflammatory response by rooibos. Overall, our data showed that aqueous rooibos extract attenuated LPS-induced liver injury possibly by modulating oxidative stress and suppressing pro-inflammatory cytokines formation.
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影响因子：
7.2
作者：
Awoniyi, Dolapo Olaitan;Aboua, Yapo Guillaume;Brooks, Nicole
通讯作者：
Brooks, Nicole
影响因子：
4.4
作者：
Hsieh, Chi-Hsun;Frink, Michael;Chaudry, Irshad H.
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Chaudry, Irshad H.
影响因子：
2.5
作者：
INANAMI, O;ASANUMA, T;KUWABARA, M
通讯作者：
KUWABARA, M
影响因子：
4.6
作者：
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通讯作者：
Chopra, K.
影响因子：
4.5
作者：
Kaur, Gaganjit;Tirkey, Naveen;Chopra, Kanwaljit
通讯作者：
Chopra, Kanwaljit