Induction and origin of adventitious shoots from chimeras of Brassica juncea and Brassica oleracea
Induction and origin of adventitious shoots from chimeras of Brassica juncea and Brassica oleracea
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芥菜和甘蓝嵌合体不定芽的诱导和起源
DOI：
10.1007/s00299-007-0398-4
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发表时间：
2007-07
影响因子：
6.2
通讯作者：
Zhao, Man
中科院分区：
文献类型：
--
作者：
Chen, Li-Ping;Ge, Ya-Ming;Zhang, Ming-Fang;Zhu, Xue-Yun;Ma, Sheng;Zhao, Man
芥菜（Brassica juncea）与红甘蓝（B.在我们以前的研究中人工合成的。以TCC（LI-LII-LIII）茎尖分生组织的最外层、中间层、最内层的节段和叶盘为外植体诱导不定芽。T =块茎芥菜，C =红甘蓝）嵌合体。通过组织学和分子生物学方法对芽的起源进行了分析。结果表明，在MS培养基中，随着BA浓度的增加，茎节部位不定芽的诱导率也随之增加。不同BA浓度的培养基对茎段基部不定芽的诱导率无明显差异。从节区产生的不定芽（丛生芽）大多数是TTT（芥菜-芥菜-芥菜），只有4个芽是嵌合体，这表明来自LI的芽多于来自LII和LIII的芽。所有茎段基部的芽均为CCC（Red cabage-Red cabbage- Red cabbage），表明其起源于LII或LII和LIII。BA和NAA的3种组合在叶盘边缘的再生率上存在显著差异。从叶盘边缘产生的不定芽大多数为CCC，但70个中有2个为嵌合体，表明LII或LII和LIII产生的不定芽多于LI。本研究中，所有通过再生获得的嵌合体在类型上都不同于原始外植体供体。不定芽的来源因供体植株上的起源部位而异，可能是多细胞多组织发生的。
The chimeras between tuber mustard (Brassica juncea) and red cabbage (B. oleracea) were artificially synthesized in our previous study. Adventitious shoots were induced from nodal segments and leaf discs of TCC (LI-LII-LIII, LI -the outmost layer of shoot apical meristem; LII -the middle layer; LIII -the innermost layer. T = Tuber mustard, C = Red cabbage) chimeras. The origin of the shoots was analyzed by histology and molecular biology. As a result, the frequency of adventitious shoot induction rose with the increase of BA in MS medium in the area of the nodes. However, there was no different induction frequency of adventitious shoots from nodal segment bases in media with different BA concentrations. Most adventitious shoots (clustered shoots) arising from the node area were TTT (Tuber mustard- Tuber mustard- Tuber mustard) and only 4 shoots were chimeras, which indicated that more shoots originated from LI than from LII and LIII. All shoots from nodal segment bases were CCC (Red cabbage-Red cabbage- Red cabbage), indicating that the shoots originated from LII or LII and LIII. There were significant differences in the regeneration rate in the margin of the leaf discs among the three combinations of BA and NAA. Most adventitious shoots from the margin of leaf discs were CCC but 2 out of 70 were chimeras, which indicated that more shoots originated from LII or LII and LIII than from LI. All chimeras obtained by regeneration were different from the original explant donor in type in the present study. The origin of the adventitious shoots varied with the site of origin on the donor plant, and could be multicellular and multihistogenic.
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DOI：
10.1007/978-3-662-02636-6_3
发表时间：
1990
期刊：
--
影响因子：
--
作者：
M. Marcotrigiano
通讯作者：
M. Marcotrigiano
DOI：
10.1016/0304-4211(75)90088-7
发表时间：
1975-03
期刊：
Plant Science Letters
影响因子：
--
作者：
L. G. Burk
通讯作者：
L. G. Burk
影响因子：
1.9
作者：
Ming-yin Li
通讯作者：
Ming-yin Li
影响因子：
2.7
作者：
H. Tian;M. Marcotrigiano
通讯作者：
H. Tian;M. Marcotrigiano
影响因子：
4.2
作者：
MARCOTRIGIANO, M;GOUIN, FR
通讯作者：
GOUIN, FR