Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?
Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?
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DOI：
10.1371/journal.pone.0254968
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Akagi M
中科院分区：
文献类型：
--
作者：
Tanaka H;Kakinoki R;Kaizawa Y;Yurie H;Ikeguchi R;Akagi M
先前，我们发现含有维管束的硅胶神经导管和植入骨髓间充质干细胞（BMSCs）的脱细胞异体基底层（DABLs）成功地实现了神经再生。临床使用的神经导管应具有柔韧性和可生物降解性。在本研究中，我们使用了由聚甘酸（PGA）纤维网制成的神经导管，它具有柔韧性、可生物降解和毛细血管渗透性。dabl是用化学表面活性剂来去除几乎所有的细胞碎片。第一部分对PGA管的毛细管渗透性能进行了研究。比较血管附着于管外（管外血管化管）的PGA管与血管内（管内血管化管）的PGA管对再生神经组织的毛细血管浸润情况。在毛细血管形成和神经再生方面，两组无明显差异。在第2部分中，我们使用含有等基因培养骨髓间充质干细胞的dabl （TubeC+组）、含有未植入骨髓间充质干细胞的dabl （TubeC-组）和20 mm长的新鲜自体神经移植物（Auto组）的管外血管化PGA管来桥接大鼠坐骨神经模型上20 mm的间隙。用电生理学和组织形态学方法评估三组神经再生情况。24周时，TubeC+组与Auto组的电生理参数均无显著差异，但Auto组的所有组织学参数均显著大于TubeC+组和TubeC-组，且TubeC+组的神经再生明显优于TubeC-组。移植的dabl未出现免疫排斥反应，部分移植的骨髓间充质干细胞分化为雪旺细胞样细胞，这可能促进了导管内的神经再生。本研究表明TubeC+神经导管可能成为自体神经移植的替代方法。
Previously, we showed silicone nerve conduits containing a vascular bundle and decellularized allogenic basal laminae (DABLs) seeded with bone marrow-derived mesenchymal stem cells (BMSCs) demonstrated successful nerve regeneration. Nerve conduits should be flexible and biodegradable for clinical use. In the current study, we used nerve conduits made of polyglycoric acid (PGA) fiber mesh, which is flexible, biodegradable and capillary-permeable. DABLs were created using chemical surfactants to remove almost all cell debris. In part 1, capillary infiltration capability of the PGA tube was examined. Capillary infiltration into regenerated neural tissue was compared between the PGA tube with blood vessels attached extratubularly (extratubularly vascularized tube) and that containing blood vessels intratubularly (intratubularly vascularized tube). No significant difference was found in capillary formation or nerve regeneration between these two tubes. In part 2, a 20 mm gap created in a rat sciatic nerve model was bridged using the extratubularly vascularized PGA tube containing the DABLs with implantation of isogenic cultured BMSCs (TubeC+ group), that containing the DABLs without implantation of the BMSCs (TubeC- group), and 20 mm-long fresh autologous nerve graft (Auto group). Nerve regeneration in these three groups was assessed electrophysiologically and histomorphometrically. At 24 weeks, there was no significant difference in any electrophysiological parameters between TubeC+ and Auto groups, although all histological parameters in Auto group were significantly greater than those in TubeC+ and TubeC- groups, and TubeC+ group demonstrated significant better nerve regeneration than TubeC- group. The transplanted DABLs showed no signs of immunological rejection and some transplanted BMSCs were differentiated into cells with Schwann cell-like phenotype, which might have promoted nerve regeneration within the conduit. This study indicated that the TubeC+ nerve conduit may become an alternative to nerve autograft.
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影响因子：
1.6
作者：
Gugliandolo, Agnese;Rajan, Thangavelu Soundara;Trubiani, Oriana
通讯作者：
Trubiani, Oriana
影响因子：
6.2
作者：
Ikeguchi, R;Kakinoki, R;Nakamura, T
通讯作者：
Nakamura, T
影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
2.1
作者：
BEST, TJ;MACKINNON, SE
通讯作者：
MACKINNON, SE
影响因子：
2.9
作者：
KAKINOKI, R;NISHIJIMA, N;YAMAMURO, T
通讯作者：
YAMAMURO, T