Intratracheal instillation methods and the distribution of administered material in the lung of the rat.
Intratracheal instillation methods and the distribution of administered material in the lung of the rat.
复制标题
DOI：
10.1293/tox.2014-0022
复制
发表时间：
2014-10
影响因子：
1.2
通讯作者：
Miyagawa M
中科院分区：
文献类型：
--
作者：
Hasegawa-Baba Y;Kubota H;Takata A;Miyagawa M
气管内滴注广泛用于实验动物的呼吸道毒性试验。然而，用于滴注的技术存在很大差异，因此很难比较使用不同技术获得的结果。为了检查滴注方法的效果，我们比较了不同条件下气管内滴注后测试物质在大鼠肺部的分布。大鼠在不同条件下气管内滴注0.3 mL印度墨水悬浮液：3种不同的身体约束角度，0°（仰卧水平）、45°（仰卧头向上）和90°（垂直头向上）； 2 种滴注速度，高 (40 mL/min) 和低 (4 mL/min)；以及 2 种不同的装置，一个标准的球头强饲针和一个设计用于气管内滴注的雾化微喷雾器。在这些不同条件下处理后一小时，处死大鼠，观察悬浮液在肺部的局部分布。仰卧平视或垂直平视位置的动物均未因治疗而死亡；然而，当大鼠被限制在仰卧水平位置时，除非在使用微喷雾器的高速给药条件下，否则观察到死亡。与其他条件相比，在高速滴注后，在仰卧头朝上位置的大鼠中观察到悬浮液在肺部更好的分布。这些结果表明，对保持仰卧平视位置的受试者进行高速滴注是进行气管内滴注的合适条件。
Intratracheal instillation is widely used for respiratory toxicity tests in experimental animals. However, there are wide variations in the techniques used for instillation, and it is thus difficult to compare the results obtained using different techniques. To examine the effect of instillation methods, we compared the distribution of a test substance in the lungs of rats after intratracheal instillations under various conditions. Rats received an intratracheal instillation of 0.3 mL of india ink suspension under different conditions as follows: 3 different angles of body restraint, 0° (supine horizontal), 45° (supine head up) and 90° (vertical head up); 2 instillation speeds, high (40 mL/min) and low (4 mL/min); and 2 different devices, a standard bulb-tipped gavage needle and an aerosolizing microsprayer designed for intratracheal instillation. One hour after treatment under these various conditions, rats were sacrificed, and the local distribution of the suspension in the lungs was observed. No animal restrained in the supine head-up or vertical head-up position died from the treatment; however, fatalities were observed when rats were restrained in the supine horizontal position except under high-speed dosing conditions with a microsprayer. Better distribution of the suspension in the lungs was observed in the rats restrained in the supine head-up position after instillation at high speed when compared with other conditions. These results indicated that high-speed instillation to the subject restrained in the supine head-up position is an appropriate condition for performing intratracheal instillation.
登录
查看更多内容
影响因子：
3.8
作者：
Driscoll, KE;Costa, DL;Schlesinger, RB
通讯作者：
Schlesinger, RB
影响因子：
3.5
作者：
HO, W;FURST, A
通讯作者：
FURST, A
影响因子：
8.3
作者：
PRITCHARD, JN;HOLMES, A;MORGAN, A
通讯作者：
MORGAN, A
影响因子：
8.3
作者：
BRAIN, JD;KNUDSON, DE;DAVIS, MA
通讯作者：
DAVIS, MA
DOI：
10.1164/ajrccm/146.4.831
发表时间：
1992-10-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
DORRIES, AM;VALBERG, PA
通讯作者：
VALBERG, PA