Photostimulable near-infrared persistent luminescent nanoprobes for ultrasensitive and longitudinal deep-tissue bio-imaging.
Photostimulable near-infrared persistent luminescent nanoprobes for ultrasensitive and longitudinal deep-tissue bio-imaging.
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作者：
Chuang YJ;Zhen Z;Zhang F;Liu F;Mishra JP;Tang W;Chen H;Huang X;Wang L;Chen X;Xie J;Pan Z
在体荧光成像存在信噪比不佳和探测深度浅的问题，这是由于在恒定的外部激励下，组织自身荧光较强，以及短波长光在组织中的散射和吸收造成的。在这里，我们通过使用一种新型的光学纳米探针，光激励LiGa5O8：Cr3+近红外（NIR）持久发光纳米粒子，它具有非常持久的NIR持久发光和独特的光激励持久发光（PSPL）的能力，允许光学成像进行无激发，因此，自发荧光的方式来解决这些限制。通过紫外光预充电的LiGa5O8：Cr3+纳米颗粒可以在体内重复（>20次）刺激，即使在深层组织中，也可以通过白色发光二极管手电筒的短照射（约15秒）产生多个NIR PSPL，将跟踪窗口从几个小时扩展到10天以上。我们的研究揭示了这些纳米探针在细胞跟踪和肿瘤靶向方面的潜力，表现出远远超过传统荧光成像所提供的卓越灵敏度和穿透力。
In vivo fluorescence imaging suffers from suboptimal signal-to-noise ratio and shallow detection depth, which is caused by the strong tissue autofluorescence under constant external excitation and the scattering and absorption of short-wavelength light in tissues. Here we address these limitations by using a novel type of optical nanoprobes, photostimulable LiGa5O8:Cr3+ near-infrared (NIR) persistent luminescence nanoparticles, which, with very-long-lasting NIR persistent luminescence and unique photo-stimulated persistent luminescence (PSPL) capability, allow optical imaging to be performed in an excitation-free and hence, autofluorescence-free manner. LiGa5O8:Cr3+ nanoparticles pre-charged by ultraviolet light can be repeatedly (>20 times) stimulated in vivo, even in deep tissues, by short-illumination (~15 seconds) with a white light-emitting-diode flashlight, giving rise to multiple NIR PSPL that expands the tracking window from several hours to more than 10 days. Our studies reveal promising potential of these nanoprobes in cell tracking and tumor targeting, exhibiting exceptional sensitivity and penetration that far exceed those afforded by conventional fluorescence imaging.
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影响因子：
4.6
作者：
Liu, Feng;Yan, Wuzhao;Chuang, Yen-Jun;Zhen, Zipeng;Xie, Jin;Pan, Zhengwei
通讯作者：
Pan, Zhengwei
影响因子：
3.4
作者：
Gao J;Chen X;Cheng Z
通讯作者：
Cheng Z
DOI：
10.1126/science.1168683
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shu X;Royant A;Lin MZ;Aguilera TA;Lev-Ram V;Steinbach PA;Tsien RY
通讯作者：
Tsien RY
DOI：
10.1073/pnas.0702427104
发表时间：
2007-05-29
影响因子：
11.1
作者：
de Chermont, Quentin le Masne;Chaneac, Corinne;Scherman, Daniel
通讯作者：
Scherman, Daniel
影响因子：
3.2
作者：
Panchuk-Voloshina, N;Haugland, RP;Haugland, RP
通讯作者：
Haugland, RP