Interfacial Biomaterial-Dentin Bacterial Biofilm Proliferation and Viability Is Affected by the Material, Aging Media and Period.
Interfacial Biomaterial-Dentin Bacterial Biofilm Proliferation and Viability Is Affected by the Material, Aging Media and Period.
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DOI：
10.3390/dj10030033
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发表时间：
2022-02-24
影响因子：
2.6
通讯作者：
Finer Y
中科院分区：
文献类型：
--
作者：
Marashdeh MQ;Lévesque C;Friedman S;Stewart CA;Finer Y
生物材料-牙本质界面随着时间的推移而降解，从而允许唾液、组织液和细菌在牙根填充物或修复物与牙本质之间移动。本研究旨在研究模拟人类唾液/细菌/血液酯酶（SHSE）中老化对四种用于填充/修复牙髓间隙的材料的牙本质界面内粪肠球菌生物膜增殖和活力的影响。制备人前牙的根管，并用牙胶和以下物质之一填充：自固化树脂复合材料（BisfilTM 2B，Bisco，绍姆堡，IL，美国），（EasyBond）或全蚀刻（TE）（ScotchbondTM，3 M，Saint Paul，MN，USA）甲基丙烯酸酯基粘合剂、环氧树脂粘合剂（AH Plus®，Dentsply Sirona，约克，PA，USA），或生物陶瓷密封剂（EndoSequence® BC Sealer™，Cheler USA，萨凡纳，GA，USA）。将标本在SHSE或磷酸盐缓冲盐水（PBS）中老化长达360天，然后培养稳态E。粪生物膜通过共聚焦激光扫描显微镜和活/死染色评估界面细菌生物膜增殖的深度和活力。采用三因素方差分析和Scheffe事后分析对数据进行分析。各材料组生物膜增殖的初始深度相似（p > 0.05）。所有组均显示出随着老化时间的增加而显著更深的生物膜增殖（p < 0.05）。SHSE老化增加TE、SE和BC的界面生物膜深度（p < 0.05），但不增加AH。对于未老化的界面，BC表现出最低的活细菌比率，其次是AH、TE和SE（p < 0.05）。界面细菌生物膜的增殖和活力取决于生物材料，老化介质和时间。
Biomaterial–dentin interfaces undergo degradation over time, allowing salivary, tissue fluid, and bacterial movement between the root filling or restoration and dentin. This study aims to investigate the effect of aging in simulated human salivary/bacterial/blood esterases (SHSE) on proliferation and viability of Enterococcus faecalis biofilm within the dentin interface with four materials used to fill/restore the endodontic space. Root canals of human anterior teeth were prepared and filled with gutta-percha and one of the following: self-cured resin composite (BisfilTM 2B, Bisco, Schaumburg, IL, USA) with either self-etch (SE) (EasyBond) or total-etch (TE) (ScotchbondTM, 3M, Saint Paul, MN, USA) methacrylate-based adhesives, epoxy-resin sealer (AH Plus®, Dentsply Sirona, York, PA, USA), or bioceramic sealer (EndoSequence® BC Sealer™, Brasseler USA, Savannah, GA, USA). Specimens were aged in SHSE or phosphate-buffered saline (PBS) for up to 360 days, followed by cultivation of steady-state E. faecalis biofilm. Depth and viability of interfacial bacterial biofilm proliferation were assessed by confocal laser scanning microscopy and live/dead staining. Data were analyzed using three-way ANOVA and Scheffe’s post hoc analyses. Initial depths of biofilm proliferation were similar among material groups (p > 0.05). All groups showed significantly deeper biofilm proliferation with increased aging period (p < 0.05). SHSE aging increased interfacial biofilm depth for TE, SE and BC (p < 0.05) but not AH. For unaged interfaces, BC exhibited the lowest ratio of live bacteria, followed by AH, TE, and SE (p < 0.05). Interfacial bacterial biofilm proliferation and viability were dependent on the biomaterial, aging media, and period.
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影响因子：
--
作者：
Gomes, Brenda Paula Figueiredo de Almeida;Pedroso, José Assis;Souza-Filho, Francisco José de
通讯作者：
Souza-Filho, Francisco José de
DOI：
10.4103/0972-0707.53338
发表时间：
2009-01-01
期刊：
Journal of conservative dentistry : JCD
影响因子：
--
作者：
Attam, Kanika;Talwar, Sangeeta;Miglani, Sanjay
通讯作者：
Miglani, Sanjay
影响因子：
5
作者：
McMichen, FRS;Pearson, G;Gulabivala, K
通讯作者：
Gulabivala, K
影响因子：
5
作者：
Marashdeh, M. Q.;Gitalis, R.;Finer, Y.
通讯作者：
Finer, Y.
影响因子：
4.2
作者：
Siqueira, JF;Rôças, IN;de Uzeda, M
通讯作者：
de Uzeda, M