High-throughput cell and particle characterization using isodielectric separation.
High-throughput cell and particle characterization using isodielectric separation.
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DOI：
10.1021/ac8019575
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发表时间：
2009-04-01
影响因子：
7.4
通讯作者：
Voldman, J.
中科院分区：
文献类型：
--
作者：
Vahey, M. D.;Voldman, J.
分离可以广泛地分为制备型和分析型，制备型的目的是从复杂的混合物中提取纯化的样品，分析型的目的是确定和量化原始混合物的含量。在这里，我们展示了一种新的微流体分离方法，等介电分离（IDS），涉及细胞和粒子跨越几个数量级的体积和电导率的分析分离范围的应用。在IDS中，细胞沿沿着电导率梯度扩散集中到其极化率消失的区域;通过测量该位置-等介电点（IDP）-随着操作条件（例如所施加电场的频率和电压）的变化，我们能够对具有不同IDP的细胞或颗粒进行分选，同时表征其电特性。我们应用这种技术来测量聚苯乙烯微球的电性能，活细胞和非活细胞的芽殖酵母酿酒酵母，和小鼠前B细胞，包括这些电性能如何随周围溶剂的电导率而变化。
Separations can be broadly categorized as preparative, where the objective is to extract purified quantities of a sample from a complex mixture, or analytic, where the goal is to determine and quantify the contents of the original mixture. Here we demonstrate the application of a new microfluidic separation method, iso-dielectric separation (IDS), to a range of analytic separations involving cells and particles spanning several orders of magnitude in volume and electrical conductivity. In IDS, cells are dielectrophoretically concentrated to the region along an electrical conductivity gradient where their polarizability vanishes; by measuring this position – the iso-dielectric point (IDP) – as operating conditions such as the frequency and voltage of the applied electric field are varied, we are able to sort cells or particles with distinct IDPs while simultaneously characterizing their electrical properties. We apply this technique to measure the electrical properties of polystyrene microspheres, viable and non-viable cells of the budding yeast Saccharomyces cerevisiae, and murine pro B cells, including how these electrical properties vary with the electrical conductivity of the surrounding solvent.
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DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
4.6
作者：
HOBURG, JF;MELCHER, JR
通讯作者：
MELCHER, JR
影响因子：
3.4
作者：
CARSTENSEN, EL;MARQUIS, RE
通讯作者：
MARQUIS, RE
影响因子：
6.1
作者：
Gawad, S;Schild, L;Renaud, P
通讯作者：
Renaud, P
影响因子：
4.4
作者：
Gascoyne, PRC;Wang, XB;Becker, FF
通讯作者：
Becker, FF