SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation.
SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation.
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DOI：
10.1093/plphys/kiac241
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发表时间：
2022-08-01
期刊：
影响因子：
7.4
通讯作者：
Ma, Ligeng
中科院分区：
文献类型：
--
作者：
Li, Ruiqi;Wei, Zhifeng;Li, Yan;Shang, Xudong;Cao, Ying;Duan, Liusheng;Ma, Ligeng
茎尖分生组织（SAM）在胚胎发生过程中形成，在植物生命周期中产生叶、茎和花器官。 SAM 发育受 SHOOT MERISTEMLESS (STM) 控制，STM 是一种保守的 I 类 KNOX 转录因子，与另一个亚类同源域蛋白 BELL 相互作用形成异二聚体，在拟南芥 (Arabidopsis thaliana) 中调节转录水平的基因表达。与此同时，SKI相互作用蛋白（SKIP）是真核生物中的保守蛋白，在植物中既充当剪接因子又充当转录调节因子，通过与不同的伙伴相互作用来控制转录和转录后水平的基因表达。在这里，我们发现，与 STM 功能丧失的植物类似，SKIP 功能丧失或 SAM 区域中 SKIP 的特异性敲除会导致 SAM 发育失败，并且突变体无法完成其生命周期。相比之下，在 SAM 区域特异性表达 SKIP 的拟南芥突变体形成了正常的 SAM，并且能够产生芽系统，包括叶和花器官。进一步的分析证实 SKIP 与植物中的 STM 相互作用，并且 SKIP 和 STM 通过与启动子结合来调节一组相似基因的表达。此外，STM还与聚合酶相关因子1复合物的组成部分EARLY FLOWERING 7 (ELF7)相互作用，ELF7的突变表现出与STM和SKIP相似的SAM缺陷。这项工作鉴定了 STM 转录复合物的一个组成部分，并揭示了拟南芥中 SKIP 介导的 SAM 形成的机制。 SKI-INTERACTING PROTEIN 与转录因子 SHOOT MERISTEMLESS 相互作用，调节芽顶端分生组织的形成和维持。
The shoot apical meristem (SAM), which is formed during embryogenesis, generates leaves, stems, and floral organs during the plant life cycle. SAM development is controlled by SHOOT MERISTEMLESS (STM), a conserved Class I KNOX transcription factor that interacts with another subclass homeodomain protein, BELL, to form a heterodimer, which regulates gene expression at the transcriptional level in Arabidopsis (Arabidopsis thaliana). Meanwhile, SKI-INTERACTING PROTEIN (SKIP), a conserved protein in eukaryotes, works as both a splicing factor and as a transcriptional regulator in plants to control gene expression at the transcriptional and posttranscriptional levels by interacting with distinct partners. Here, we show that, similar to plants with a loss of function of STM, a loss of function of SKIP or the specific knockout of SKIP in the SAM region resulted in failed SAM development and the inability of the mutants to complete their life cycle. In comparison, Arabidopsis mutants that expressed SKIP specifically in the SAM region formed a normal SAM and were able to generate a shoot system, including leaves and floral organs. Further analysis confirmed that SKIP interacts with STM in planta and that SKIP and STM regulate the expression of a similar set of genes by binding to their promoters. In addition, STM also interacts with EARLY FLOWERING 7 (ELF7), a component of Polymerase-Associated Factor 1 complex, and mutation in ELF7 exhibits similar SAM defects to that of STM and SKIP. This work identifies a component of the STM transcriptional complex and reveals the mechanism underlying SKIP-mediated SAM formation in Arabidopsis. SKI-INTERACTING PROTEIN interacts with transcription factor SHOOT MERISTEMLESS to regulate shoot apical meristem formation and maintenance.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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