Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.
Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.
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DOI：
10.1016/j.antiviral.2023.105651
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发表时间：
2023-08
影响因子：
7.6
通讯作者：
中科院分区：
文献类型：
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作者：
Many poxviruses are significant human and animal pathogens, including viruses that cause smallpox and mpox (formerly monkeypox). Identifying novel and potent antiviral compounds is critical to successful drug development targeting poxviruses. Here we tested two compounds, nucleoside trifluridine, and nucleotide adefovir dipivoxil, for antiviral activities against vaccinia virus (VACV), mpox virus (MPXV), and cowpox virus (CPXV) in physiologically relevant primary human fibroblasts. Both compounds potently inhibited the replication of VACV, CPXV, and MPXV (MA001 2022 isolate) in plaque assays. In our recently developed assay based on a recombinant VACV expressing secreted Gaussia luciferase, they both exhibited high potency in inhibiting VACV replication with EC50s in the low nanomolar range. In addition, both trifluridine and adefovir dipivoxil inhibited VACV DNA replication and downstream viral gene expression. Our results characterized trifluridine and adefovir dipivoxil as strong poxvirus antiviral compounds and further validate the VACV Gaussia luciferase assay as a highly efficient and reliable reporter tool for identifying poxvirus inhibitors. Given that both compounds are FDA-approved drugs, and trifluridine is already used to treat ocular vaccinia, further development of trifluridine and adefovir dipivoxil holds great promise in treating poxvirus infections, including mpox.
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影响因子：
3.1
作者：
Hughes, Laura;Wilkins, Kimberly;Goldsmith, Cynthia S.;Smith, Scott;Hudson, Paul;Patel, Nishi;Karem, Kevin;Damon, Inger;Li, Yu;Olson, Victoria A.;Satheshkumar, P. S.
通讯作者：
Satheshkumar, P. S.
影响因子：
6.4
作者：
Altmann, Sharon;Brandt, Curtis R.;Kodihalli, Shantha
通讯作者：
Kodihalli, Shantha
DOI：
10.3390/v2091933
发表时间：
2010-09
期刊：
Viruses
影响因子：
--
作者：
Hendrickson RC;Wang C;Hatcher EL;Lefkowitz EJ
通讯作者：
Lefkowitz EJ
影响因子：
56.3
作者：
Adler, Hugh;Gould, Susan;Hine, Paul;Snell, Luke B.;Wong, Waison;Houlihan, Catherine F.;Osborne, Jane C.;Rampling, Tommy;Beadsworth, Mike Bj;Duncan, Christopher Ja;Dunning, Jake;Fletcher, Tom E.;Hunter, Ewan R.;Jacobs, Michael;Khoo, Saye H.;Newsholme, William;Porter, David;Porter, Robert J.;Ratcliffe, Libuse;Schmid, Matthias L.;Semple, Malcolm G.;Tunbridge, Anne J.;Wingfield, Tom;Price, Nicholas M.
通讯作者：
Price, Nicholas M.
影响因子：
1
作者：
Holec AD;Mandal S;Prathipati PK;Destache CJ
通讯作者：
Destache CJ