Chlamydia trachomatis persistence in vitro: an overview.
Chlamydia trachomatis persistence in vitro: an overview.
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DOI：
10.1086/652394
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发表时间：
2010-06-15
期刊：
影响因子：
--
通讯作者：
Wyrick PB
中科院分区：
文献类型：
--
作者：
Wyrick PB
在体外目标粘膜人上皮细胞中生长的衣原体可以从其正常的发育周期进程-在感染性但代谢不活跃的初级体（EB）到代谢活性但非感染性的网状体（RB）之间交替过渡到EB -进入一种“持久性”状态。体外持久性被定义为“有活力但不可培养的衣原体”，包括“形态扩大，异常，不分裂的RB”。这种情况是“可逆的”，在去除诱导剂后产生感染性EB，包括青霉素、干扰素、铁/营养饥饿、伴发疱疹感染或宿主细胞成熟到其生理分化状态。由于衣原体基因组的上调/下调和随后的宿主反应不同，所有异常RB表型都不相同。虽然所有持续性诱导条件都存在于体内，但关键问题包括：(i)在感染患者和动物中存在的急性-沉默型慢性-急性衣原体感染交替过程中，体内是否会发生异常衣原体RB，以及（ii）这种异常衣原体RB是否会导致长期慢性炎症、纤维化和瘢痕形成。
Chlamydiae growing in target mucosal human epithelial cells in vitro can transition from their normal developmental cycle progression -- alternating between infectious but metabolically-inactive elementary bodies (EB) to metabolically-active but non-infectious reticulate bodies (RB) and back to EB -- into a state of “persistence”. Persistence in vitro is defined as “viable but non-cultivable chlamydiae” involving “morphologically enlarged, aberrant, non-dividing RB”. The condition is “reversible” to yield infectious EB on removal of the inducers, including penicillin, interferon-gamma, iron/nutrient starvation, concomitant herpes infection, or maturation of the host cell into its physiologically-differentiated state. All aberrant RB phenotypes are not the same due to differing up/down regulated chlamydial gene sets and subsequent host responses. While all persistence-inducing conditions exist in vivo, key questions include whether or not (i) aberrant chlamydial RB occur in vivo during the alternating acute-silent chronic-acute chlamydial infection scenario that exists in infected patients and animals and (ii) if such aberrant RB can contribute to prolonged, chronic inflammation, fibrosis and scarring .
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