Unilateral Intervention in the Sinuses of Rabbits Induces Bilateral Inflammatory and Microbial Changes.
Unilateral Intervention in the Sinuses of Rabbits Induces Bilateral Inflammatory and Microbial Changes.
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DOI：
10.3389/fcimb.2021.585625
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发表时间：
2021
影响因子：
5.7
通讯作者：
Biswas K
中科院分区：
文献类型：
--
作者：
Lux CA;Johnston JJ;Waldvogel-Thurlow S;Dassi C;Douglas RG;Cho DY;Taylor MW;Biswas K
慢性鼻窦炎（CRS）是一种全球流行的鼻窦炎性疾病，严重影响患者的生活质量。先前已经描述了通过暂时性窦闭塞在兔中建立单侧鼻窦炎的动物模型。本研究的目的是通过观察细菌群落和粘膜炎症的时间和双侧变化来建立家兔鼻窦炎模型。通过内窥镜将Merocel®（一种无菌鼻腔填塞材料）放置在6只新西兰白色家兔的左中鼻道中4周，实现鼻窦炎的发展。总共14周后，通过内窥镜检查、磁共振成像（MRI）和组织学评估家兔的鼻窦炎。在研究期间的三个时间点（第0周、第4周、第14周），从左中鼻道和右中鼻道采集拭子用于细菌群落分析。内镜评价显示，在4周阻断期后和第14周检查的所有动物均出现单侧炎症。值得注意的是，第4周所有动物的对侧鼻窦中也观察到炎症变化。MRI图像显示2只家兔在第4周时出现单侧窦混浊，仅1只家兔在第14周时出现部分单侧窦混浊。组织学分析显示，所有动物的粘膜炎症具有显著的空间异质性，结果不一致。在第14周时，鼻孔之间的粘膜炎症标志物（如杯状细胞增生、上皮剥脱和水肿）无显著差异。在第0周（基线），兔鼻窦中的细菌群落主要由螺杆菌主导。在封闭期结束时（第4周），两个鼻孔中的细菌α和β多样性显著增加。第14周的细菌群落组成基本恢复至基线水平，反映了内镜和放射学结果。本研究再次证实了在家兔模型中不接种任何病原体的情况下发生鼻窦炎的能力。我们能够证明双边鼻窦粘膜炎症，通过诱导单侧鼻窦阻塞，导致鼻窦细菌群落的显着变化。这些发现可以解释CRS中的一些临床观察结果，并需要进一步研究以揭示其治疗管理的潜在意义。
Chronic rhinosinusitis (CRS) is a globally prevalent inflammatory condition of the paranasal sinuses which severely impairs patients’ quality of life. An animal model of unilateral sinusitis by transient sinus occlusion has been described previously in rabbits. The aim of this study was to characterise the sinusitis rabbit model by investigating temporal and bilateral changes in the bacterial community and mucosal inflammation. Development of sinusitis was achieved by endoscopically placing Merocel®, a sterile nasal packing material, in the left middle meatus of six New Zealand white rabbits for four weeks. After a total period of 14 weeks, rabbits were assessed for sinusitis by endoscopic examination, magnetic resonance imaging (MRI) and histology. Swabs from the left and right middle meatus were obtained for bacterial community analysis at three time points (week 0, week 4, week 14) during the study. Endoscopic evaluation showed unilateral inflammation in all animals examined after the 4-week blocking period and at week 14. Notably, inflammatory changes were also seen in the contralateral sinus of all animals at week 4. MRI images demonstrated unilateral sinus opacification at week 4 in two rabbits, and partial unilateral sinus opacification at week 14 in one rabbit only. Histological analyses revealed substantial spatial heterogeneity of mucosal inflammation with inconsistent findings across all animals. No significant differences in mucosal inflammatory markers (such as goblet cell hyperplasia, epithelial denudation and oedema) could be identified between nostrils at week 14. The bacterial community in the rabbit sinuses was heavily dominated by Helicobacter at week 0 (baseline). At the end of the blocking period (week 4), bacterial alpha and beta diversity were significantly increased in both nostrils. The bacterial community composition at week 14 had primarily returned to baseline, reflecting the endoscopic and radiological results. This study reaffirmed the ability for development of sinusitis without inoculation of any pathogens in a rabbit model. We were able to demonstrate bilateral sinonasal mucosal inflammation, by inducing unilateral sinus blockage, which resulted in significant changes to the sinonasal bacterial community. These findings may explain some of the clinical observations seen in CRS and warrant further research to reveal potential implications for its therapeutic management.
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影响因子：
4.6
作者：
Chalermwatanachai T;Vilchez-Vargas R;Holtappels G;Lacoere T;Jáuregui R;Kerckhof FM;Pieper DH;Van de Wiele T;Vaneechoutte M;Van Zele T;Bachert C
通讯作者：
Bachert C
DOI：
10.2500/ajr.2007.21.3032
发表时间：
2007-05-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Ha, Kien R.;Psaltis, Alkis J.;Wormald, Peter-John
通讯作者：
Wormald, Peter-John
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Zwetsloot, Casper P.
通讯作者：
Zwetsloot, Casper P.
影响因子：
3.7
作者：
Boase S;Foreman A;Cleland E;Tan L;Melton-Kreft R;Pant H;Hu FZ;Ehrlich GD;Wormald PJ
通讯作者：
Wormald PJ
影响因子：
16.6
作者：
Abolins S;King EC;Lazarou L;Weldon L;Hughes L;Drescher P;Raynes JG;Hafalla JCR;Viney ME;Riley EM
通讯作者：
Riley EM