Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry.

Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry.
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DOI:
10.1021/jp900266k
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发表时间:
2009-03-24
期刊:
The journal of physical chemistry. C, Nanomaterials and interfaces
影响因子:
--
通讯作者:
Van Duyne RP
Van Duyne RP
中科院分区:
其他
文献类型:
--
作者:
Anker JN;Hall WP;Lambert MP;Velasco PT;Mrksich M;Klein WL;Van Duyne RP

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首次将高分辨率局域表面等离子体共振(HR-LSPR)传感器与基质辅助激光解吸电离质谱(MALDI-MS)联用。LSPR传感器提供分子吸附的实时无标记检测。随后的MALDI-MS分析能够鉴定吸附的分子。这种协同LSPR-MS方法被应用于检测和鉴定淀粉样蛋白β寡聚体,其在阿尔茨海默病的分子发病机制中起重要作用。
High resolution localized surface plasmon resonance (HR-LSPR) sensors were combined with matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) for the first time. LSPR sensors provide real-time label-free detection of molecular adsorption. Subsequent MALDI-MS analysis enables identification of the adsorbed molecules. This synergistic LSPR-MS approach was applied to the detection and identification of amyloid beta oligomers which play an important role in the molecular pathogenesis of Alzheimer’s Disease.
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