The metabolic footprint of Clostridia and Erysipelotrichia reveals their role in depleting sugar alcohols in the cecum.
The metabolic footprint of Clostridia and Erysipelotrichia reveals their role in depleting sugar alcohols in the cecum.
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DOI：
10.1186/s40168-021-01123-9
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发表时间：
2021-08-19
期刊：
影响因子：
15.5
通讯作者：
Bäumler AJ
中科院分区：
文献类型：
--
作者：
Tiffany CR;Lee JY;Rogers AWL;Olsan EE;Morales P;Faber F;Bäumler AJ
微生物群的分解代谢活动通过帮助营养、免疫教育和针对病原体的生态位保护而有助于健康。然而，肠道微生物群中常见分类群消耗的营养素仍然不完全清楚。在这里，我们将微生物群分析与非靶向代谢组学方法相结合，以确定小鼠盲肠中小代谢物的消耗是否与特定细菌分类群的存在相关。通过将复杂或确定的微生物群落植入无菌或驱虫处理的小鼠来研究因果关系。我们注意到，由抗生素治疗引发的肠道微生物群中梭菌属和丹毒丝菌属的消耗与糖酸和糖醇（多元醇）的盲肠浓度增加有关。值得注意的是，当我们用14种梭菌属和3种丹毒毛菌属分离株的确定的微生物群落接种无菌小鼠时，我们观察到相反的情况，盲肠内容物中糖酸和多元醇的浓度显著降低。由确定的微生物群落产生的碳水化合物足迹与在接受来自常规小鼠的盲肠微生物群移植的无菌小鼠中观察到的相似。补充山梨糖醇，多元醇用作人工甜味剂，增加盲肠山梨糖醇的浓度，这是废除接种后，梭菌分离能够生长在山梨糖醇在体外。我们得出结论，梭菌和丹毒菌属消耗糖醇消耗这些代谢产物从肠腔在稳态。视频摘要在线版本包含补充材料，可在10. 1186/s40168-021-01123-9。
The catabolic activity of the microbiota contributes to health by aiding in nutrition, immune education, and niche protection against pathogens. However, the nutrients consumed by common taxa within the gut microbiota remain incompletely understood. Here we combined microbiota profiling with an un-targeted metabolomics approach to determine whether depletion of small metabolites in the cecum of mice correlated with the presence of specific bacterial taxa. Causality was investigated by engrafting germ-free or antibiotic-treated mice with complex or defined microbial communities. We noted that a depletion of Clostridia and Erysipelotrichia from the gut microbiota triggered by antibiotic treatment was associated with an increase in the cecal concentration of sugar acids and sugar alcohols (polyols). Notably, when we inoculated germ-free mice with a defined microbial community of 14 Clostridia and 3 Erysipelotrichia isolates, we observed the inverse, with a marked decrease in the concentrations of sugar acids and polyols in cecal contents. The carbohydrate footprint produced by the defined microbial community was similar to that observed in gnotobiotic mice receiving a cecal microbiota transplant from conventional mice. Supplementation with sorbitol, a polyol used as artificial sweetener, increased cecal sorbitol concentrations in antibiotic-treated mice, which was abrogated after inoculation with a Clostridia isolate able to grow on sorbitol in vitro. We conclude that consumption of sugar alcohols by Clostridia and Erysipelotrichia species depletes these metabolites from the intestinal lumen during homeostasis. Video abstract The online version contains supplementary material available at 10.1186/s40168-021-01123-9.
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影响因子：
29.4
作者：
BEAUGERIE, L;FLOURIE, B;RAMBAUD, JC
通讯作者：
RAMBAUD, JC
影响因子：
30.3
作者：
Jacobson A;Lam L;Rajendram M;Tamburini F;Honeycutt J;Pham T;Van Treuren W;Pruss K;Stabler SR;Lugo K;Bouley DM;Vilches-Moure JG;Smith M;Sonnenburg JL;Bhatt AS;Huang KC;Monack D
通讯作者：
Monack D
DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP