As a biomarker for gastric cancer, circPTPN22 regulates the progression of gastric cancer through the EMT pathway.
As a biomarker for gastric cancer, circPTPN22 regulates the progression of gastric cancer through the EMT pathway.
复制标题
作为胃癌的生物标志物，circPTPN22通过EMT通路调节胃癌的进展
DOI：
10.1186/s12935-020-01701-1
复制
发表时间：
2021-01-11
影响因子：
5.8
通讯作者：
Ju S
中科院分区：
文献类型：
--
作者：
Ma S;Kong S;Gu X;Xu Y;Tao M;Shen L;Shen X;Ju S
胃癌是世界上最常见的恶性肿瘤之一。由于缺乏特异性症状，80%以上的患者被诊断为晚期，死亡率高，因此早期诊断GC非常重要。环状RNA（CircRNA）是一类结构稳定、半衰期长、具有肿瘤特异性的内源性非编码RNA。它可以用作肿瘤的诊断标志物。利用circRNA测序技术对3对胃癌及癌旁组织进行筛选，筛选出表达差异显著的circRNA。通过RT-qPCR、琼脂糖凝胶电泳、桑格测序、RNase R和放线菌素D测定来确定circPTPN 22的环状结构和特征。采用Cell Counting Kit-8、集落形成、Transwell、伤口愈合、小鼠成瘤及Western blotting等方法检测circPTPN 22对胃癌细胞体外增殖、侵袭、迁移、肿瘤生长及蛋白表达的影响。CircPTPN 22在GC组织、细胞和血浆中上调并与转移正相关。RT-qPCR结果显示，circPTPN 22对胃癌有较好的诊断效果，可用于预测胃癌患者的预后。体内外实验表明，下调circPTPN 22可通过上皮-间充质转化（EMT）途径抑制细胞增殖、迁移和侵袭。CircPTPN 22可能通过竞争性结合miRNA来调节GC进展。CircPTPN 22可作为胃癌的潜在诊断和预后标志物，并可通过竞争性结合miRNA抑制EMT通路来抑制细胞增殖和转移。
Gastric cancer (GC) is one of the most common cancers in the world. Due to the lack of specific symptoms, more than 80% of patients are diagnosed as the advanced stage with a high mortality rate, so the early diagnosis of GC is incredibly essential. Circular RNAs (CircRNAs) are a kind of endogenous non-coding RNA with stable structure, the long half-life, and tumor specificity. It can be used as a diagnostic marker for tumors. Using circRNA sequencing technology screened three pairs of GC and adjacent tissues, and circRNAs with significant expression differences were screened out. The circular structure and characteristics of circPTPN22 were determined by RT-qPCR, agarose gel electrophoresis, Sanger sequencing, RNase R, and actinomycin D assays. Cell Counting Kit‐8, colony formation, Transwell, Wound healing, tumor formation in mice and western blotting assays were used to detect the effects of circPTPN22 on the proliferation, invasion, migration, tumor growth of GC cells in vitro and protein expression. CircPTPN22 is up-regulated and positively correlated with metastasis in GC tissues, cells, and plasma. RT-qPCR results showed that circPTPN22 had good diagnostic efficacy and could be used to predict the prognosis of GC patients. In vitro and vivo experiments showed that the downregulation of circPTPN22 could inhibit cell proliferation, migration, and invasion through the epithelial-mesenchymal transformation (EMT) pathway. CircPTPN22 may regulate GC progression through the competitive binding of miRNAs. CircPTPN22 can be used as a potential diagnostic and prognostic marker for GC and can inhibit cell proliferation and metastasis through the competitive binding of miRNA to inhibit the EMT pathway.
登录
查看更多内容
影响因子：
14.9
作者：
Enuka Y;Lauriola M;Feldman ME;Sas-Chen A;Ulitsky I;Yarden Y
通讯作者：
Yarden Y
影响因子：
4.6
作者：
Barrett, Steven P.;Salzman, Julia
通讯作者：
Salzman, Julia
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
新型环状RNA circFN1通过海绵miR-182-5p增强胃癌顺铂耐药性
影响因子：
4
作者：
Huang, Xiao-xu;Zhang, Qiang;Xu, Li
通讯作者：
Xu, Li
circHMCU 通过支持 let-7 家族促进乳腺癌的增殖和转移
影响因子：
8.8
作者：
Song, Xiaojin;Liang, Yiran;Yang, Qifeng
通讯作者：
Yang, Qifeng