Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.
Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.
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建立可持续的珊瑚细胞系，鹿角珊瑚。
DOI：
10.1007/s10126-021-10031-w
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Satoh N
中科院分区：
文献类型：
--
作者：
Kawamura K;Nishitsuji K;Shoguchi E;Fujiwara S;Satoh N
石珊瑚的浮游幼虫，Acropora tenuis，由细长的外胚层细胞和发育中的内胚层细胞组成。为了建立体外细胞系用于珊瑚细胞的细胞和发育潜力的未来研究，通过用由胰蛋白酶、EDTA和胶原酶组成的组织解离溶液处理幼虫，成功地将其解离成单细胞。褐色细胞、半透明细胞和淡蓝色细胞是游离幼虫的主要组成部分。棕色细胞在为珊瑚设计的培养基中开始短暂增殖，而半透明细胞和淡蓝色细胞在细胞解离后约1周数量减少。此外，当一个模块化的蛋白酶，纤溶酶，被添加到细胞培养基中，棕色的细胞延长伪足和假定无定形形状。然后，它们继续成团增殖超过6个月，倍增时间约为4-5天。从细胞培养3周开始，棕色细胞经常聚集并表现出形态发生样行为，形成扁平片和囊胚样簇或原肠胚样球。通过RNA-seq分析细胞系的单细胞或细胞簇。分析表明，这些细胞中表达的基因在体外是A。tenuis基因此外，每个细胞系表达一组特定的基因，表明它们的特性包括胃胚层、分泌细胞、未分化细胞、神经元细胞和表皮。在连续的细胞培养过程中，所有细胞特性均保持稳定。这些结果证实了珊瑚体外细胞系的成功建立。在线版本包含补充材料，可通过10.1007/s10126-021-10031-w获得。
Planula larvae of the scleractinian coral, Acropora tenuis, consist of elongated ectodermal cells and developing inner endodermal cells. To establish in vitro cell lines for future studies of cellular and developmental potential of coral cells, larvae were successfully dissociated into single cells by treating them with a tissue dissociation solution consisting of trypsin, EDTA, and collagenase. Brown-colored cells, translucent cells, and pale blue cells were the major components of dissociated larvae. Brown-colored cells began to proliferate transiently in the culture medium that was devised for the coral, while translucent cells and pale blue cells decreased in number about 1 week after cell dissociation. In addition, when a modular protease, plasmin, was added to the cell culture medium, brown-colored cells extended pseudopodia and assumed amorphous shapes. They then continued to proliferate in clumps for more than 6 months with a doubling time of approximately 4–5 days. From 3 weeks of cell culture onward, brown-colored cells often aggregated and exhibited morphogenesis-like behavior to form flat sheets, and blastula-like clusters or gastrula-like spheres. Single cells or cell-clusters of the cell lines were analyzed by RNA-seq. This analysis showed that genes expressed in these cells in vitro were A. tenuis genes. Furthermore, each cell line expressed a specific set of genes, suggesting that their properties include gastroderm, secretory cells, undifferentiated cells, neuronal cells, and epidermis. All cell properties were maintained stably throughout successive cell cultures. These results confirm the successful establishment of a coral in vitro cell line. The online version contains supplementary material available at 10.1007/s10126-021-10031-w.
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影响因子：
3.7
作者：
Mass T;Drake JL;Haramaty L;Rosenthal Y;Schofield OM;Sherrell RM;Falkowski PG
通讯作者：
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影响因子：
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作者：
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影响因子：
2.4
作者：
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通讯作者：
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14.8
作者：
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通讯作者：
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影响因子：
2.4
作者：
Hayward, DC;Miller, DJ;Ball, EE
通讯作者：
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