RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3K/Akt Signaling Mediated by IGF-I.
RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3K/Akt Signaling Mediated by IGF-I.
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DOI：
10.1093/ecam/nep216
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Huang CY
中科院分区：
文献类型：
--
作者：
Chang YM;Kuo WH;Lai TY;Shih YT;Tsai FJ;Tsai CH;Shu WT;Chen YY;Chen YS;Kuo WW;Huang CY
雪旺细胞的增殖对于受损神经的再生至关重要。地龙在中草药中被广泛用于清除瘀血和促进伤口愈合的功能。这种中草药究竟是如何促进组织存活的尚不清楚。本研究旨在探讨地龙促进神经元再生的分子机制。我们的研究结果表明，地龙提取物处理诱导胰岛素样生长因子-I（IGF-I）介导的磷脂酰肌醇3-激酶/丝氨酸-苏氨酸激酶（PI 3 K/Akt）途径的磷酸化，并激活细胞核抗原（PCNA）的蛋白表达，在一个时间依赖性的方式。细胞周期分析显示，苦参提取物作用12-16 h后，细胞由G1期进入S期，20 h后S期进入G2期。细胞周期蛋白D1、细胞周期蛋白E和细胞周期蛋白A的强表达以时间依赖性方式发生。小干扰RNA（siRNA）介导的PI 3 K敲低显着降低PI 3 K蛋白表达水平，导致Bcl 2生存因子减少，并显着阻断增殖细胞中G1至S的转变。这些结果表明地龙通过IGF-I信号通路促进RSC 96细胞的增殖和存活。其机制主要依赖于PI 3 K蛋白。
Schwann cell proliferation is critical for the regeneration of injured nerves. Dilongs are widely used in Chinese herbal medicine to remove stasis and stimulate wound-healing functions. Exactly how this Chinese herbal medicine promotes tissue survival remains unclear. The aim of the present study was to investigate the molecular mechanisms by which Dilong promote neuron regeneration. Our results show that treatment with extract of Dilong induces the phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3-kinase/serine-threonine kinase (PI3K/Akt) pathway, and activates protein expression of cell nuclear antigen (PCNA) in a time-dependent manner. Cell cycle analysis showed that G1 transits into the S phase in 12–16 h, and S transits into the G2 phase 20 h after exposure to earthworm extract. Strong expression of cyclin D1, cyclin E and cyclin A occurs in a time-dependent manner. Small interfering RNA (siRNA)-mediated knockdown of PI3K significantly reduced PI3K protein expression levels, resulting in Bcl2 survival factor reduction and a marked blockage of G1 to S transition in proliferating cells. These results demonstrate that Dilong promotes the proliferation and survival of RSC96 cells via IGF-I signaling. The mechanism is mainly dependent on the PI3K protein.
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DOI：
10.1093/ecam/neh094
发表时间：
2005-06
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cooper EL
通讯作者：
Cooper EL
DOI：
10.1093/ecam/neh002
发表时间：
2004-06-01
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cooper EL
通讯作者：
Cooper EL
影响因子：
5.1
作者：
Balamurugan, Mariappan;Parthasarathi, Kasi;Cooper, Edwin L.
通讯作者：
Cooper, Edwin L.
影响因子：
7.8
作者：
CARONI, P;GRANDES, P
通讯作者：
GRANDES, P
影响因子：
4
作者：
GONG, JG;MCBRIDE, D;WEBB, R
通讯作者：
WEBB, R