Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in wild strawberry.
Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in wild strawberry.
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DOI：
10.1093/jxb/ery384
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发表时间：
2019-01-07
影响因子：
6.9
通讯作者：
Kang C
中科院分区：
文献类型：
--
作者：
Feng J;Dai C;Luo H;Han Y;Liu Z;Kang C
由DR 5ver 2：：GUS揭示的生长素分布和由GUS报告基因确定的FveTAR和FveYUC的表达模式强调了胚乳是草莓瘦果中主要的生长素生物合成位点。生长素在草莓座果和花托膨大中的关键作用已被证实。虽然已知受精触发生长素的生物合成，但受精诱导的生长素的特定组织来源还不清楚。在此，将生长素报告基因DR 5ver 2：：GUS导入野生草莓（Fragaria vesca）以揭示受精前后种子和果实托以及根中生长素的分布。此外，使用与β-葡萄糖醛酸酶（GUS）报告基因融合的TAR和YUCCA各自的启动子研究了编码催化两步生长素生物合成途径的酶的TAR和YUCCA基因的表达。两个FveTAR和四个FveYUC被证明主要在胚乳和瘦果内的胚以及根尖和侧根原基中表达。这些报告者在解剖组织中的表达提供了更详细和精确的空间（细胞和组织）和时间（受精前和受精后）的信息，生长素的合成和积累比以前的研究在草莓。此外，我们产生了种子中最丰富的YUC FveYUC 10的CRISPR介导的敲除突变体;突变体在幼果中具有较低的游离生长素水平，但没有显示出明显的形态表型。然而，FveYUC 10的过表达导致拟南芥中生长素水平升高引起的下胚轴伸长。总的来说，这项研究揭示了生长素积累的合点端种皮，胚，花托脉管系统，根尖，侧根原基，并强调了胚乳作为主要的生长素生物合成网站座果。
Auxin distribution revealed by DR5ver2::GUS and expression patterns of FveTARs and FveYUCs determined by the GUS reporter highlighted the endosperm as the main auxin biosynthesis site in strawberry achenes. The critical role of auxin in strawberry fruit set and receptacle enlargement was demonstrated previously. While fertilization is known to trigger auxin biosynthesis, the specific tissue source of fertilization-induced auxin is not well understood. Here, the auxin reporter DR5ver2::GUS was introduced into wild strawberry (Fragaria vesca) to reveal auxin distribution in the seed and fruit receptacle pre- and post-fertilization as well as in the root. In addition, the expression of TAR and YUCCA genes coding for enzymes catalysing the two-step auxin biosynthesis pathway was investigated using their respective promoters fused to the β-glucuronidase (GUS) reporter. Two FveTARs and four FveYUCs were shown to be expressed primarily in the endosperm and embryo inside the achenes as well as in root tips and lateral root primordia. Expression of these reporters in dissected tissues provided more detailed and precise spatial (cell and tissue) and temporal (pre- and post-fertilization) information on where auxin is synthesized and accumulates than previous studies in strawberry. Moreover, we generated CRISPR-mediated knock-out mutants of FveYUC10, the most abundant YUC in seeds; the mutants had a lower free auxin level in young fruit, but displayed no obvious morphological phenotypes. However, overexpression of FveYUC10 resulted in elongated hypocotyls in Arabidopsis caused by elevated auxin level. Overall, the study revealed auxin accumulation in the chalazal seed coat, embryo, receptacle vasculature, root tip, and lateral root primordia and highlighted the endosperm as the main auxin biosynthesis site for fruit set.
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影响因子：
7.4
作者：
Hollender, Courtney A.;Kang, Chunying;Liu, Zhongchi
通讯作者：
Liu, Zhongchi
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde
影响因子：
5.6
作者：
Estrada-Johnson E;Csukasi F;Pizarro CM;Vallarino JG;Kiryakova Y;Vioque A;Brumos J;Medina-Escobar N;Botella MA;Alonso JM;Fernie AR;Sánchez-Sevilla JF;Osorio S;Valpuesta V
通讯作者：
Valpuesta V
影响因子：
48
作者：
Liao, Che-Yang;Smet, Wouter;Weijers, Dolf
通讯作者：
Weijers, Dolf