Correlation of expression of BP1, a homeobox gene, with estrogen receptor status in breast cancer.
Correlation of expression of BP1, a homeobox gene, with estrogen receptor status in breast cancer.
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DOI：
10.1186/bcr602
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Berg PE
中科院分区：
文献类型：
--
作者：
Fu SW;Schwartz A;Stevenson H;Pinzone JJ;Davenport GJ;Orenstein JM;Gutierrez P;Simmens SJ;Abraham J;Poola I;Stephan DA;Berg PE
BP1是本实验室克隆的一个新的同源异型盒基因。我们以前在白血病中的研究表明，BP1具有致癌特性，包括作为细胞存活的调节剂。在这里，我们检测了乳腺癌中BP1的表达，并确定了BP1表达与临床病理数据之间的关系。从细胞系、肿瘤和匹配的正常邻近组织或尸检组织中分离总RNA。逆转录聚合酶链反应检测BP1的表达。用SAS软件进行统计学分析。对46例浸润性导管乳腺肿瘤的分析表明，其中80%的肿瘤中BP1表达，而6例正常乳腺组织中BP1表达缺失，1例正常乳腺组织中BP1表达水平较低。值得注意的是，100%的雌激素受体（ER）阴性的肿瘤是BP1阳性的，而73%的ER阳性肿瘤表达BP1（P = 0.03）。BP1表达也与种族有关：89%的非裔美国妇女的肿瘤是BP1阳性，而57%的白人妇女表达BP1（P = 0.04）。然而，BP1的表达在I级、II级和III级肿瘤之间没有显著差异。有趣的是，BP1 mRNA表达与恶性细胞系在小鼠中引起乳腺癌的能力相关。由于BP1在乳腺肿瘤中表达异常，因此它可以为治疗提供有用的靶点，特别是在ER阴性肿瘤患者中。BP1在所有肿瘤分级中的频繁表达表明BP1的激活是早期事件。
BP1 is a novel homeobox gene cloned in our laboratory. Our previous studies in leukemia demonstrated that BP1 has oncogenic properties, including as a modulator of cell survival. Here BP1 expression was examined in breast cancer, and the relationship between BP1 expression and clinicopathological data was determined. Total RNA was isolated from cell lines, tumors, and matched normal adjacent tissue or tissue from autopsy. Reverse transcription polymerase chain reaction was performed to evaluate BP1 expression. Statistical analysis was accomplished with SAS. Analysis of 46 invasive ductal breast tumors demonstrated BP1 expression in 80% of them, compared with a lack of expression in six normal breast tissues and low-level expression in one normal breast tissue. Remarkably, 100% of tumors that were negative for the estrogen receptor (ER) were BP1-positive, whereas 73% of ER-positive tumors expressed BP1 (P = 0.03). BP1 expression was also associated with race: 89% of the tumors of African American women were BP1-positive, whereas 57% of those from Caucasian women expressed BP1 (P = 0.04). However, there was no significant difference in BP1 expression between grades I, II, and III tumors. Interestingly, BP1 mRNA expression was correlated with the ability of malignant cell lines to cause breast cancer in mice. Because BP1 is expressed abnormally in breast tumors, it could provide a useful target for therapy, particularly in patients with ER-negative tumors. The frequent expression of BP1 in all tumor grades suggests that activation of BP1 is an early event.
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影响因子：
56.9
作者：
GEHRING, WJ
通讯作者：
GEHRING, WJ
影响因子：
64.5
作者：
LEVINE, M;RUBIN, GM;TJIAN, R
通讯作者：
TJIAN, R
影响因子：
2.2
作者：
Mancino, AT;Rubio, IT;Klimberg, VS
通讯作者：
Klimberg, VS
DOI：
10.1073/pnas.91.6.2156
发表时间：
1994-03-15
影响因子：
11.1
作者：
KALLIONIEMI, A;KALLIONIEMI, OP;WALDMAN, FM
通讯作者：
WALDMAN, FM
影响因子：
3.5
作者：
Fu, SD;Stevenson, H;Berg, PE
通讯作者：
Berg, PE