Alterations in the gut microbiota and metabolite profiles of patients with Kashin-Beck disease, an endemic osteoarthritis in China.
Alterations in the gut microbiota and metabolite profiles of patients with Kashin-Beck disease, an endemic osteoarthritis in China.
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大骨节病（中国的一种地方性骨关节炎）患者肠道微生物群和代谢物谱的变化。
DOI：
10.1038/s41419-021-04322-2
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发表时间：
2021-10-28
影响因子：
9
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Wang X;Ning Y;Li C;Gong Y;Huang R;Hu M;Poulet B;Xu K;Zhao G;Zhou R;Lammi MJ;Guo X
大骨节病（KBD）是一种严重的骨软骨病，可能是由遗传和环境因素之间的相互作用驱动的。我们的目的是提高我们对不同级别的大骨节病患者的肠道菌群结构以及肠道菌群与血清代谢产物之间的关系的理解。从大骨节病患者和正常对照（NC）收集的粪便和血清样品用于通过液相色谱-质谱（LC/MS）使用16 S rDNA基因和代谢组测序来表征肠道微生物群。为了确定物种水平上的肠道微生物变化是否与大骨节病患者肠道细菌的基因或功能相关，对I级大骨节病、II级大骨节病和NC受试者的粪便样本进行了宏基因组测序。大骨节病组的特征是梭杆菌和拟杆菌的水平升高。共鉴定出56个属在两组之间具有显著差异丰度。大骨节病群中Alloprevotella、Robinsoniella、Megamonas和Escherichia_Shigella属较为丰富。与属水平的16 S rDNA分析一致，根据宏基因组测序，大骨节病受试者中的大多数差异丰度物种属于普雷沃氏菌属。血清代谢组学分析确定了I级和II级大骨节病和NC组中参与脂质代谢代谢网络的一些差异丰富的代谢物，如不饱和脂肪酸和甘油磷脂。此外，我们发现代谢物水平的这些差异与特定物种丰度的改变有关。我们的研究提供了大骨节病患者肠道微生物群和代谢物的全面景观，并为大骨节病发病机制中肠道微生物组和代谢组之间的新型相互作用提供了大量证据。
Kashin-Beck disease (KBD) is a severe osteochondral disorder that may be driven by the interaction between genetic and environmental factors. We aimed to improve our understanding of the gut microbiota structure in KBD patients of different grades and the relationship between the gut microbiota and serum metabolites. Fecal and serum samples collected from KBD patients and normal controls (NCs) were used to characterize the gut microbiota using 16S rDNA gene and metabolomic sequencing via liquid chromatography-mass spectrometry (LC/MS). To identify whether gut microbial changes at the species level are associated with the genes or functions of the gut bacteria in the KBD patients, metagenomic sequencing of fecal samples from grade I KBD, grade II KBD and NC subjects was performed. The KBD group was characterized by elevated levels of Fusobacteria and Bacteroidetes. A total of 56 genera were identified to be significantly differentially abundant between the two groups. The genera Alloprevotella, Robinsoniella, Megamonas, and Escherichia_Shigella were more abundant in the KBD group. Consistent with the 16S rDNA analysis at the genus level, most of the differentially abundant species in KBD subjects belonged to the genus Prevotella according to metagenomic sequencing. Serum metabolomic analysis identified some differentially abundant metabolites among the grade I and II KBD and NC groups that were involved in lipid metabolism metabolic networks, such as that for unsaturated fatty acids and glycerophospholipids. Furthermore, we found that these differences in metabolite levels were associated with altered abundances of specific species. Our study provides a comprehensive landscape of the gut microbiota and metabolites in KBD patients and provides substantial evidence of a novel interplay between the gut microbiome and metabolome in KBD pathogenesis.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
16.6
作者：
Boer, Cindy G.;Radjabzadeh, Djawad;van Meurs, Joyce B. J.
通讯作者：
van Meurs, Joyce B. J.
影响因子：
7
作者：
Cao, J.;Li, S.;Caterson, B.
通讯作者：
Caterson, B.
蛋白多糖代谢、细胞死亡和大骨节病
影响因子：
3
作者：
Li, Siyuan;Cao, Junling;Caterson, Bruce;Hughes, Clare E.
通讯作者：
Hughes, Clare E.
膳食营养失衡以及相关的差异表达基因和通路可能在幼年卡申-贝克病中发挥重要作用
DOI：
10.1016/j.jtemb.2018.01.012
发表时间：
2018-01-01
影响因子：
3.5
作者：
Ning, Yujie;Wang, Xi;Guo, Xiong
通讯作者：
Guo, Xiong