Heavy metal-immobilizing bacteria combined with calcium polypeptides reduced the uptake of Cd in wheat and shifted the rhizosphere bacterial communities.
Heavy metal-immobilizing bacteria combined with calcium polypeptides reduced the uptake of Cd in wheat and shifted the rhizosphere bacterial communities.
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重金属固定细菌与钙多肽结合减少了小麦对镉的吸收，并改变了根际细菌群落。
DOI：
10.1016/j.envpol.2020.115432
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发表时间：
2020-08
影响因子：
8.9
通讯作者：
Zhao-jin Chen
中科院分区：
文献类型：
--
作者：
Hui Han;Xue-jiao Wu;Lun-guang Yao;Zhao-jin Chen
原位稳定技术对重金属污染土壤的“修复”是一种新颖而廉价的技术。然而，外源钝化剂与小麦根际土壤细菌群落相互作用的机制尚不清楚。通过土壤静态培养和盆栽试验，研究了布甘肠杆菌（enterobacter bugandenensij6）和钙多肽（CPPs）对小麦镉吸收、土壤质量和根际细菌群落结构的影响和机制。结果表明，与对照处理（CK）相比，TJ6、CPP和TJ6+CPP处理显著降低了小麦根际土壤中二乙烯三胺五乙酸（DTPA）可提取Cd含量（25.2% ~ 60.1%），提高了pH、有机质含量和脲酶活性，导致小麦组织（籽粒、秸秆和根系）Cd含量（21.5% ~ 77.8%）降低。特别是TJ6+CPP处理在降低籽粒Cd积累方面更为有效。此外，TJ6+CPP处理提高了小麦根际土壤细菌群落的多样性，proteobacteria、Firmicutes、Arthrobacter、Microvirga、Ensifer、Brevundimonas、devosiaand pedobacter相对丰度增加。这些结果表明，TJ6+CPP处理通过以下途径降低了小麦对Cd的吸收：1)提供必需元素（N和C源），2)提高了小麦根际土壤pH值，降低了小麦根际土壤生物可利用Cd含量，3)允许定植促进植物生长和Cd抗性细菌，4)增加了根际细菌群落中ABC转运体、碳代谢和氧化磷酸化相关基因的丰度。结果表明，重金属固定化菌TJ6与CPPs联合施用降低了小麦根际土壤Cd含量，增加了土壤细菌群落多样性。我们的研究结果还强调了利用重金属固定化细菌和CPPs来确保重金属污染土壤上作物安全生产的潜力。
In situ stabilization techniques for the “remediation” of heavy metal-contaminated soil are a novel and inexpensive technology. However, the mechanisms underlying the interaction of exogenous passivators with the bacterial community in wheat rhizosphere soil remain unclear. Soil static culture and pot experiments were conducted to evaluate the effects and mechanisms of the heavy metal-immobilizing bacteriumEnterobacter bugandensisTJ6 and calcium polypeptides (CPPs) and their association with Cd uptake in wheat, soil quality and the rhizobacterial community structure. The results showed that compared with the control treatment (CK), the TJ6, CPP, and TJ6+CPP treatments significantly decreased the diethylenetriaminepentaacetic acid (DTPA)-extractable Cd (25.2%–60.1%) content and increased the pH, organic matter content and urease activity in the wheat rhizosphere soil, which resulted in decreases in the Cd (21.5%–77.8%) content in wheat tissues (grain, straw, and roots). In particular, the TJ6+CPP treatment was more effective at decreasing Cd accumulation in grains. Furthermore, the TJ6+CPP treatment improved the diversity of the soil bacterial community in the wheat rhizosphere, and the relative abundances ofProteobacteria,Firmicutes,Arthrobacter,Microvirga,Ensifer,Brevundimonas,DevosiaandPedobacterwere enriched. These results suggest that the TJ6+CPP treatment decreased the uptake of Cd in wheat by i) providing essential elements (N and C sources), ii) increasing the pH and reducing the bioavailable Cd content in wheat rhizosphere soil, iii) allowing colonization to promote plant growth and Cd-resistant bacteria, and iv) increasing the abundance of genes associated with ABC transporters, carbon metabolism and oxidative phosphorylation in the rhizosphere bacterial community. Our results showed that the heavy metal-immobilizing bacterium TJ6 combined with CPPs decreased the Cd content and increased the bacterial community diversity of wheat rhizosphere soil. Our results also highlight the potential of using heavy metal-immobilizing bacteria and CPPs to ensure the safe production of crops growing on heavy metal-polluted soils.
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影响因子：
4.8
作者：
Hidri, Rabaa;Metoui-Ben Mahmoud, Ouissal;Azcon, Rosario
通讯作者：
Azcon, Rosario
pH 对胶束增强超滤废水中重金属形态和去除的影响
影响因子：
8.8
作者：
Huang, Jinhui;Yuan, Fang;Shi, Yahui
通讯作者：
Shi, Yahui
影响因子：
13.6
作者：
P. Yu;Youping Sun;Zhongliang Huang;F. Zhu;Yujing Sun;Lijuan Jiang
通讯作者：
P. Yu;Youping Sun;Zhongliang Huang;F. Zhu;Yujing Sun;Lijuan Jiang
DOI：
10.1016/j.scitotenv.2020.139972
发表时间：
2020-06
期刊：
The Science of the total environment
影响因子：
--
作者：
Cheng Cheng-Cheng;Hui Han;Yaping Wang;Ru Wang;Linyan He;X. Sheng
通讯作者：
Cheng Cheng-Cheng;Hui Han;Yaping Wang;Ru Wang;Linyan He;X. Sheng
影响因子：
8.9
作者：
Hou, Dandi;Wang, Runze;Tian, Shengke
通讯作者：
Tian, Shengke