Chemical Properties of Human Dentin Blocks and Vertical Augmentation by Ultrasonically Demineralized Dentin Matrix Blocks on Scratched Skull without Periosteum of Adult-Aged Rats.
Chemical Properties of Human Dentin Blocks and Vertical Augmentation by Ultrasonically Demineralized Dentin Matrix Blocks on Scratched Skull without Periosteum of Adult-Aged Rats.
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DOI：
10.3390/ma15010105
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发表时间：
2021-12-24
期刊：
影响因子：
--
通讯作者：
Murata M
中科院分区：
文献类型：
--
作者：
Zhu B;Yokozeki K;Kabir MA;Todoh M;Akazawa T;Murata M
垂直增强是骨工程中最具挑战性的技术之一。几个参数，如机械化学特性，是重要的优化垂直骨再生利用生物材料。本研究的目的是化学表征人类牙本质块（钙化脱矿牙本质基质：CDM，部分脱矿牙本质基质：PDDM和完全脱矿牙本质基质：CDDM） （2 × 2 × 1 mm3）的化学特征，并评估PDDM块作为垂直增强模型在未刮伤或刮伤无骨膜的成年大鼠（10-12月龄，雌性）颅骨上的行为。在1.0 L 2% HNO3溶液中超声脱矿30 min后，CDM的溶解率为32.3%。脱矿30 min的牙本质命名为PDDM。PDDM的SEM图像显示牙本质管开口，纳米微裂纹，表面光滑。在胶原酶消化试验中，12周时CDM、PDDM和CDDM的减重率分别为9.2%、25.5%和78.3%。与PDDM和CDDM相比，CDM抑制胶原酶消化。在超声划伤颅骨上的PDDM嵌合移植物中，在4周和8周时，在成年老年大鼠PDDM块与致密颅骨之间的骨桥形成中发现原骨的骨髓间隙开口。另一方面，在非划伤颅骨和划伤颅骨的骨髓空间闭合的情况下，骨桥没有形成。结果表明，超声刮入致密的顶骨可促进颅骨骨髓间隙的打开和骨髓细胞的供应，从而在无骨膜的垂直增强模型中形成骨桥。
Vertical augmentation is one of the most challenging techniques in bone engineering. Several parameters, such mechano-chemical characteristics, are important to optimize vertical bone regeneration using biomaterials. The aims of this study were to chemically characterize human dentin blocks (calcified demineralized dentin matrix: CDM, partially demineralized dentin matrix: PDDM and completely demineralized dentin matrix: CDDM) (2 × 2 × 1 mm3) chemically and evaluate the behavior of PDDM blocks on non-scratched or scratched skulls without periosteum of adult rats (10–12 months old, female) as a vertical augmentation model. The dissolved efficiency of CDM showed 32.3% after ultrasonic demineralization in 1.0 L of 2% HNO3 for 30 min. The 30 min-demineralized dentin was named PDDM. The SEM images of PDDM showed the opening of dentinal tubes, nano-microcracks and the smooth surface. In the collagenase digestion test, the weight-decreasing rates of CDM, PDDM and CDDM were 9.2%, 25.5% and 78.3% at 12 weeks, respectively. CDM inhibited the collagenase digestion, compared with PDDM and CDDM. In the PDDM onlay graft on an ultrasonically scratched skull, the bone marrow-space opening from original bone was found in the bony bridge formation between the human PDDM block and dense skull of adult senior rats at 4 and 8 weeks. On the other hand, in the cases of the marrow-space closing in both non-scratched skulls and scratched skulls, the bony bridge was not formed. The results indicated that the ultrasonic scratching into the compact parietal bone might contribute greatly to the marrow-space opening from skull and the supply of marrow cells, and then bony bridge formation could occur in the vertical augmentation model without a periosteum.
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影响因子：
4.9
作者：
Murata, Masaru;Sato, Daisuke;Arisue, Makoto
通讯作者：
Arisue, Makoto
影响因子：
2.5
作者：
Qin, Xu;Raj, Regmi M.;Zhou, Bin
通讯作者：
Zhou, Bin
DOI：
10.1016/s0030-4220(81)80005-9
发表时间：
1981-01-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
AMES, JR;RYAN, DE;MAKI, KA
通讯作者：
MAKI, KA
影响因子：
3.7
作者：
Koga T;Minamizato T;Kawai Y;Miura K;I T;Nakatani Y;Sumita Y;Asahina I
通讯作者：
Asahina I
影响因子：
0.4
作者：
Akazawa, Toshiyuki;Murata, Masaru;Kimura, Isao
通讯作者：
Kimura, Isao