The Arabidopsis LRR-RLK, PXC1, is a regulator of secondary wall formation correlated with the TDIF-PXY/TDR-WOX4 signaling pathway.
The Arabidopsis LRR-RLK, PXC1, is a regulator of secondary wall formation correlated with the TDIF-PXY/TDR-WOX4 signaling pathway.
复制标题
拟南芥 LRR-RLK，PXC1，是与 TDIF-PXY/TDR-WOX4 信号通路相关的次生壁形成的调节因子
DOI：
10.1186/1471-2229-13-94
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发表时间：
2013-07-01
影响因子：
5.3
通讯作者：
Zheng B
中科院分区：
文献类型：
--
作者：
Wang J;Kucukoglu M;Zhang L;Chen P;Decker D;Nilsson O;Jones B;Sandberg G;Zheng B
背景
尽管在植物中发现了许多富含亮氨酸重复序列的受体样蛋白编码基因(LRR-RLKs)，但只有少数基因的功能被确定。最近的研究表明，LRR-RLK，PXY/TDR，在血管二次发育过程中起重要作用。其他研究表明，PXY/TDR不太可能是参与这一复杂过程的唯一LRR-RLK。
结果
在这项研究中，电子分析导致鉴定了三个拟南芥LRR-RLK基因(PXY相关；PXC1，2，3)，它们的转录积累谱与几个关键的维管发育调控因子密切相关，包括PXY/TDR，HB-8，REV和CLE41。使用qPCR和启动子的表达谱：报告线表明，所有三个PXC基因都与维管系统相关。其中PXC1(At2g36570)与PXY/TDR有很强的相关性。将pxc1突变体从长日照转移到短日照表明，该基因的缺失导致木质部纤维次生壁的形成显著减少。对包括PXY/TDR在内的多种次生细胞壁相关基因的转录本分析表明，PXC1介导的途径与TDIF-PXY/TDR-WOX4系统介导的途径相连。
结论
结果表明，LRR-RLK、PXC1参与了木质部纤维次生细胞壁的形成。虽然还需要进一步的研究来确定PXC1蛋白的配体和作用方式，但从这项工作中可以清楚地看出，类似于茎顶端分生组织(SAM)，次生维管发育需要许多LRR-RLK的贡献。
Background
Although a number of leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs) have been identified in plants, a functional role has been determined for only a few. Recent studies have demonstrated that an LRR-RLK, PXY/TDR, is important for the process of secondary vascular development. Other studies have indicated that PXY/TDR is unlikely to be the sole LRR-RLK involved in this complex process.
Results
In this study, in silico analyses led to the identification of three Arabidopsis LRR-RLK genes (PXY-correlated; PXC1, 2, 3) with transcript accumulation profiles that correlated strongly with several key regulators of vascular development, including PXY/TDR, HB-8, REV, and CLE41. Expression profiling using qPCR and promoter:reporter lines indicated that all three PXC genes are associated with the vasculature. One in particular, PXC1 (At2g36570), had a strong correlation with PXY/TDR. Shifting pxc1 mutants from long-days to short-days showed that loss of the gene led to a dramatic reduction in secondary wall formation in xylem fibers. Transcript analysis of mutants for a variety of secondary cell wall-associated genes, including PXY/TDR indicated that the pathways mediated by PXC1 connect with those mediated by the TDIF-PXY/TDR-WOX4 system.
Conclusions
The data indicate that the LRR-RLK, PXC1 is involved in secondary cell wall formation in xylem fibers. Whereas further study is needed to identify the ligands and mode of action of the PXC1 protein, it is clear from this work that similarly to the shoot apical meristem (SAM), secondary vascular development requires contributions from a number of LRR-RLKs.
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