MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis.
MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis.
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DOI：
10.1007/s10495-018-1474-7
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Zhao D
中科院分区：
文献类型：
--
作者：
Gao J;Kong R;Zhou X;Ji L;Zhang J;Zhao D
miR-126和IL-23 R均影响类风湿关节炎（RA）的进程。本研究旨在探讨miR-126与IL-23 R的相关性以及miR-126对RA发病机制的可能调控作用。收集RA患者血清、滑膜组织和滑液，检测miR-126、IL-23 R、TNF-α和IFN-γ的表达。采用胶原诱导的关节炎小鼠模型建立成纤维样滑膜细胞（FLS）。使用编码pro-miR-126和anti-miR-126的慢病毒质粒或miR-126激动剂和相应的阴性对照手动干预miR-126的表达。与对照组相比，RA患者miR-126的表达受到抑制（P < 0.05）。RA患者TNF-α、IFN-γ的产生及IL-23 R的表达均显著高于对照组（P < 0.05）。在经pro-miR-126处理的FLS细胞中，给予pro-miR-126质粒上调miR-126，但抑制IL-23 R、TNF-α和IFN-γ的表达或产生。此外，miR-126激动剂逆转了抗miR-126质粒对FLS的影响。这些结果表明miR-126负调控IL-23 R、TNF-α和IFN-γ的表达。这些结果表明miR-126对RA进程的关键影响。此外，pro-miR-126有可能成为治疗RA的潜在药物。
Both miR-126 and IL-23R affect rheumatoid arthritis (RA) procession. This study aimed to investigate the association of miR-126 and IL-23R and the possible modulation of miR-126 to RA pathogenesis. Serum, synovial tissue and synovial fluid were collected from patients with RA, and expression of miR-126, IL-23R, TNF-α and IFN-γ were detected. Fibroblast-like synoviocytes (FLS) was established using a collagen-induced arthritis mice model. The expression of miR-126 was manual intervened using pro-miR-126 and anti-miR-126 encoding lentivirus plasmids, or miR-126 agonists and corresponding negative controls. MiR-126 expression was inhibited in RA patients when compared with controls (P < 0.05). TNF-α and IFN-γ production and IL-23R expression were significantly upregulated in RA patients when compared to controls (P < 0.05). In pro-miR-126 treated FLS cells, the administration of pro-miR-126 plasmids upregulated miR-126, but inhibited IL-23R, TNF-α and IFN-γ expression or production. Moreover, the miR-126 agonist reversed the effects of the anti-miR-126 plasmid on FLS. These results revealed that miR-126 negative regulated the expression of IL-23R, TNF-α and IFN-γ. These results suggest the key impact of miR-126 on RA procession. Moreover, pro-miR-126 might be explored to be a potential therapy for RA.
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影响因子：
30.5
作者：
通讯作者：
--
波兰风湿关节炎患者的IL-17A，IL-17F和IL-23R基因多态性。
影响因子：
3.2
作者：
Bogunia-Kubik, Katarzyna;Swierkot, Jerzy;Malak, Anna;Wysoczanska, Barbara;Nowak, Beata;Bialowas, Katarzyna;Gebura, Katarzyna;Korman, Lucyna;Wiland, Piotr
通讯作者：
Wiland, Piotr
DOI：
10.4049/jimmunol.1203172
发表时间：
2013-05-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Duhen R;Glatigny S;Arbelaez CA;Blair TC;Oukka M;Bettelli E
通讯作者：
Bettelli E
DOI：
10.1084/jem.20030896
发表时间：
2003-12-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Murphy CA;Langrish CL;Chen Y;Blumenschein W;McClanahan T;Kastelein RA;Sedgwick JD;Cua DJ
通讯作者：
Cua DJ
MicroRNA-126通过靶向PIK3R2并调节PI3K-AKT信号通路影响类风湿性关节炎滑膜成纤维细胞增殖和凋亡
影响因子：
--
作者：
Qu Y;Wu J;Deng JX;Zhang YP;Liang WY;Jiang ZL;Yu QH;Li J
通讯作者：
Li J