Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice.
Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal cortex in mice.
复制标题
小鼠内侧前额叶皮质光遗传学自我刺激过程中的全脑动力学。
DOI：
10.1038/s42003-020-01612-x
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发表时间：
2021-01-14
影响因子：
5.9
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Cover CG;Kesner AJ;Ukani S;Stein EA;Ikemoto S;Yang Y;Lu H
颅内自我刺激是一种被广泛用于研究动机行为的程序，在这种刺激中，动物进行操作性反应以接受局部脑电刺激。虽然长期以来，局部神经元活动的测量是在手术前后立即进行的，但实时成像整个大脑仍然是一个挑战。在这里，我们报告了一种方法，该方法允许在提示小鼠执行操作任务时进行脑动力学的功能磁共振(FMRI)：在提示开启但不提示关闭的过程中，舔嘴以接受对内侧前额叶皮质(MPFC)的光遗传刺激。在提示时舔会激活与潜在MPFC投射一致的广泛分布的网络，而在提示关闭时舔(没有光遗传刺激)会导致与急性灭绝相关的大脑区域的fMRI信号为负。非侵入性的全脑读数与电路特定的神经调节相结合，为研究健康和疾病模型中的适应行为开辟了一条途径。Cover等人。开发一种方法，在提示小鼠执行一项可操作的任务时，允许对大脑动力学进行功能磁共振成像：舔嘴，以接受对内侧前额叶皮质的光遗传刺激。这种方法提供了非侵入性的全脑读数，用于小鼠对电路特定神经调节的适应行为。
Intracranial self-stimulation, in which an animal performs an operant response to receive regional brain electrical stimulation, is a widely used procedure to study motivated behavior. While local neuronal activity has long been measured immediately before or after the operant, imaging the whole brain in real-time remains a challenge. Herein we report a method that permits functional MRI (fMRI) of brain dynamics while mice are cued to perform an operant task: licking a spout to receive optogenetic stimulation to the medial prefrontal cortex (MPFC) during a cue ON, but not cue OFF. Licking during cue ON results in activation of a widely distributed network consistent with underlying MPFC projections, while licking during cue OFF (without optogenetic stimulation) leads to negative fMRI signal in brain regions involved in acute extinction. Noninvasive whole brain readout combined with circuit-specific neuromodulation opens an avenue for investigating adaptive behavior in both healthy and disease models. Cover et al. develop a method that permits functional MRI of brain dynamics while mice are cued to perform an operant task: licking a spout to receive optogenetic stimulation to the medial prefrontal cortex. This method presents noninvasive whole brain readout for adaptive behavior of mice in response to circuit-specific neuromodulation.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
影响因子：
10.6
作者：
Heilbronner SR;Rodriguez-Romaguera J;Quirk GJ;Groenewegen HJ;Haber SN
通讯作者：
Haber SN
影响因子：
5.7
作者：
Han, Zhe;Chen, Wenjing;Liang, Zhifeng
通讯作者：
Liang, Zhifeng
影响因子：
--
作者：
BARONE, FC;DEEGAN, JF;ORMSBEE, HS
通讯作者：
ORMSBEE, HS
DOI：
10.1126/science.aac9698
发表时间：
2016-01-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ferenczi EA;Zalocusky KA;Liston C;Grosenick L;Warden MR;Amatya D;Katovich K;Mehta H;Patenaude B;Ramakrishnan C;Kalanithi P;Etkin A;Knutson B;Glover GH;Deisseroth K
通讯作者：
Deisseroth K