Stem cell proliferation versus meiotic fate decision in Caenorhabditis elegans.
Stem cell proliferation versus meiotic fate decision in Caenorhabditis elegans.
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DOI：
10.1007/978-1-4614-4015-4_4
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发表时间：
2013
影响因子：
--
通讯作者：
Schedl, Tim
中科院分区：
文献类型：
--
作者：
Hansen, Dave;Schedl, Tim
关键词：
秀丽隐杆线虫的生殖系已经成为理解干细胞群体如何在动物的整个生命周期中维持，同时仍然产生物种繁殖所需的配子的重要模型。成年雌雄同体的干细胞数量相对较大，干细胞产生的子细胞似乎本质上是相同的；然而，一些子细胞保留了增殖的命运，而另一些则进入减数分裂前期。虽然细胞通过有丝分裂细胞周期和减数分裂前期的机制存在，但了解生殖系发育的核心是确定决定有丝分裂细胞周期或减数分裂前期机制的基因和调控过程；换句话说，就是调节生殖细胞从增殖干细胞走向减数分裂发育的基因。生殖细胞是自我更新还是进入减数分裂前期，很大程度上取决于其与远端尖端细胞（DTC）的接近程度，远端尖端细胞是覆盖性腺远端的体细胞壁龛细胞。靠近DTC的生殖细胞具有高水平的GLP-1 Notch信号，促进增殖命运，而远离DTC的细胞具有高水平的GLD-1和GLD-2冗余RNA调控途径，以及第三种未表征的途径，每种途径都指导细胞进入减数分裂前期。其他因素和途径调节这一核心遗传途径，或与之并行，可能是为了确保增殖和减数分裂进入之间保持紧密的平衡。
The C. elegans germ line has emerged as an important model for understanding how a stem cell population is maintained throughout the life of the animal while still producing the gametes necessary for propagation of the species. The stem cell population in the adult hermaphrodite is relatively large, with stem cells giving rise to daughters that appear intrinsically equivalent; however, some of the daughters retain the proliferative fate while others enter meiotic prophase. While machinery exists for cells to progress through the mitotic cell cycle and machinery exists for cells to progress through meiotic prophase, central to understanding germline development is identifying the genes and regulatory processes that determine whether the mitotic cell cycle or meiotic prophase machinery will be utilized; in other words, the genes that regulate the switch of germ cells from the proliferative stem cell fate to the meiotic development fate. Whether a germ cell self-renews or enters meiotic prophase is largely determined by its proximity to the distal tip cell (DTC), which is the somatic niche cell that caps the distal end of the gonad. Germ cells close to the DTC have high levels of GLP-1 Notch signaling, which promotes the proliferative fate, while cells further from the DTC have high activity levels of the GLD-1 and GLD-2 redundant RNA regulatory pathways, as well as a third uncharacterized pathway, each of which direct cells to enter meiotic prophase. Other factors and pathways modulate this core genetic pathway, or work in parallel to it, presumably to ensure that a tight balance is maintained between proliferation and meiotic entry.
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DOI：
10.1098/rstb.2003.1333
发表时间：
2003-08-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Crittenden, SL;Eckmann, CR;Kimble, J
通讯作者：
Kimble, J
影响因子：
11.8
作者：
Eckmann, CR;Kraemer, B;Kimble, J
通讯作者：
Kimble, J
影响因子：
3.3
作者：
Crittenden, Sarah L.;Leonhard, Kimberly A.;Kimble, Judith
通讯作者：
Kimble, Judith
影响因子：
4.6
作者：
Belfiore, M;Pugnale, P;Puoti, A
通讯作者：
Puoti, A
影响因子：
64.5
作者：
AUSTIN, J;KIMBLE, J
通讯作者：
KIMBLE, J