Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.
Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.
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来自拟鼻疽伯克霍尔德菌的假定响应调节剂 BPSL0128 的接收器域的结晶和初步 X 射线分析。
DOI:
10.1107/s1744309112025791
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发表时间:
2012
期刊:
影响因子:
--
通讯作者:
Abd Aziz AG
中科院分区:
文献类型:
--
作者:
Abd Aziz AG
bpsl0128, a gene encoding a putative response regulator from Burkholderia pseudomallei strain D286, has been cloned into a pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. The full-length protein is degraded during purification to leave a fragment corresponding to the putative receiver domain, and crystals of this protein that diffracted to beyond 1.75 Å resolution have been grown by the hanging-drop vapour-diffusion technique using PEG 6000 as the precipitant. The crystals belonged to one of the enantiomorphic pair of space groups P3121 and P3221, with unit-cell parameters a = b = 65.69, c = 105.01 Å and either one or two molecules in the asymmetric unit.
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期刊:
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