Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.

Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.
复制标题

来自拟鼻疽伯克霍尔德菌的假定响应调节剂 BPSL0128 的接收器域的结晶和初步 X 射线分析。

DOI:
10.1107/s1744309112025791
复制
发表时间:
2012
期刊:
Acta crystallographica. Section F, Structural biology and crystallization communications
影响因子:
--
通讯作者:
Abd Aziz AG
Abd Aziz AG
中科院分区:
--
文献类型:
--
作者:
Abd Aziz AG

文献摘要

参考文献

相似文献

bps 10128是编码来自类鼻疽伯克霍尔德氏菌菌株D286的推定反应调节因子的基因,已被克隆到pETBLUE-1载体系统中,在大肠杆菌中过表达并纯化。全长蛋白质在纯化过程中降解,留下一个片段对应于推定的接收域,和晶体衍射超过1.75 μ m的分辨率的这种蛋白质已经生长的悬滴气相扩散技术,使用PEG 6000作为沉淀剂。 晶体属于空间群为P3121和P3221的对映体之一,晶胞参数a = B = 65.69,c = 105.01,不对称单元中有一个或两个分子。 
bpsl0128, a gene encoding a putative response regulator from Burkholderia pseudomallei strain D286, has been cloned into a pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. The full-length protein is degraded during purification to leave a fragment corresponding to the putative receiver domain, and crystals of this protein that diffracted to beyond 1.75 Å resolution have been grown by the hanging-drop vapour-diffusion technique using PEG 6000 as the precipitant. The crystals belonged to one of the enantiomorphic pair of space groups P3121 and P3221, with unit-cell parameters a = b = 65.69, c = 105.01 Å and either one or two molecules in the asymmetric unit.
DOI: 10.1016/j.diagmicrobio.2007.01.002
发表时间: 2007-07-01
影响因子: 2.9
作者:
Lee, Song-Hua;Chong, Chan-Eng;Nathan, Sheila
通讯作者: Nathan, Sheila
类鼻疽发病机制的研究现状。
DOI: --
发表时间: 1999
影响因子: 5.8
作者:
D. Woods;D. DeShazer;Richard A. Moore;P. Brett;M. Burtnick;Shauna L. Reckseidler;Michelle D Senkiw
通讯作者: Michelle D Senkiw
DOI: 10.1073/pnas.95.9.5317
发表时间: 1998-04-28
影响因子: 11.1
作者:
Barrett, JF;Goldschmidt, RM;Hoch, JA
通讯作者: Hoch, JA
DOI: 10.1086/422458
发表时间: 2004-08-15
影响因子: 11.8
作者:
Cosgrove, SE;Carroll, KC;Perl, TM
通讯作者: Perl, TM
鼻疽伯克霍尔德氏菌对宿主细胞的利用。
DOI: 10.1078/1438-4221-00292
发表时间: 2004
期刊: International journal of medical microbiology : IJMM
影响因子: --
作者:
M. Stevens;E. Galyov
通讯作者: E. Galyov