Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis.
Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis.
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患有外阴阴道念珠菌病的育龄妇女的阴道微生物群多样化
DOI：
10.1371/journal.pone.0079812
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou HW
中科院分区：
文献类型：
--
作者：
Liu MB;Xu SR;He Y;Deng GH;Sheng HF;Huang XM;Ouyang CY;Zhou HW
外阴阴道假丝酵母菌病(VVC)是最常见的阴道感染性疾病之一，有关与其相关的阴道微生物区系多样性的报道一直存在争议。我们用16S rRNA标签的Illumina测序法测定了VVC、细菌性阴道病(BV)以及VVC和BV混合感染患者的阴道微生物群落。我们的结果首次揭示了VVC患者阴道微生物组的高度变异模式。总体上，物种丰富度和均匀度的α多样性结果表现为：正常对照+lt；仅VVC+lt；混合BV和VVC感染+lt；群落结构的贝塔多样性比较也表明，对照和BV样本之间存在VVC的中间成分。详细的比较表明，尽管对照和BV群落具有典型的模式，但VVC的阴道微生物区系是复杂的。BV和VVC混合感染组表现出一种独特的模式，乳杆菌丰度高于BV组，普氏杆菌、加德纳氏菌和阿托波氏菌丰度高于正常对照组。相比之下，仅VVC组不能用任何单一的剖面来描述，从类似于正常对照的群落结构(以乳杆菌为主)到类似BV的群落结构(富含Gardnerella和Atopobium)。VVC的治疗导致阴道微生物区系的不一致变化，四个BV/VVC样本恢复到更高的乳酸杆菌水平，而许多仅VVC患者没有。这些结果将有助于进一步研究阴道微生物区系在VVC和相关传染病中的作用。
Vulvovaginal candidiasis (VVC) is one of the most prevalent vaginal infectious diseases, and there are controversial reports regarding the diversity of the associated vaginal microbiota. We determined the vaginal microbial community in patients with VVC, bacterial vaginosis (BV), and mixed infection of VVC and BV using Illumina sequencing of 16S rRNA tags. Our results revealed for the first time the highly variable patterns of the vaginal microbiome from VVC patients. In general, the alpha-diversity results of species richness and evenness showed the following order: normal control < VVC only < mixed BV and VVC infection < BV only. The beta-diversity comparison of community structures also showed an intermediate composition of VVC between the control and BV samples. A detailed comparison showed that, although the control and BV communities had typical patterns, the vaginal microbiota of VVC is complex. The mixed BV and VVC infection group showed a unique pattern, with a relatively higher abundance of Lactobacillus than the BV group and higher abundance of Prevotella, Gardnerella, and Atopobium than the normal control. In contrast, the VVC-only group could not be described by any single profile, ranging from a community structure similar to the normal control (predominated with Lactobacillus) to BV-like community structures (abundant with Gardnerella and Atopobium). Treatment of VVC resulted in inconsistent changes of the vaginal microbiota, with four BV/VVC samples recovering to a higher Lactobacillus level, whereas many VVC-only patients did not. These results will be useful for future studies on the role of vaginal microbiota in VVC and related infectious diseases.
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影响因子：
3.7
作者：
Shipitsyna E;Roos A;Datcu R;Hallén A;Fredlund H;Jensen JS;Engstrand L;Unemo M
通讯作者：
Unemo M
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影响因子：
3.7
作者：
Srinivasan S;Hoffman NG;Morgan MT;Matsen FA;Fiedler TL;Hall RW;Ross FJ;McCoy CO;Bumgarner R;Marrazzo JM;Fredricks DN
通讯作者：
Fredricks DN
影响因子：
10.5
作者：
Ma B;Forney LJ;Ravel J
通讯作者：
Ravel J
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
影响因子：
11.4
作者：
Pirotta, MV;Gunn, JM;Chondros, P
通讯作者：
Chondros, P