Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria.
Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria.
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细菌性阴道病女性的细菌群落：高分辨率系统发育分析揭示了微生物群与临床标准的关系。
DOI：
10.1371/journal.pone.0037818
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fredricks DN
中科院分区：
文献类型：
--
作者：
Srinivasan S;Hoffman NG;Morgan MT;Matsen FA;Fiedler TL;Hall RW;Ross FJ;McCoy CO;Bumgarner R;Marrazzo JM;Fredricks DN
细菌性阴道病（BV）是一种常见的疾病，与许多不良的健康结果有关，其特征是对阴道微生物群的变化知之甚少。我们试图通过16 S rRNA基因的深度测序结合物种水平的分类鉴定来描述BV女性阴道细菌生物群的组成和多样性。我们研究了单个细菌种类的存在与BV临床诊断特征之间的关系。对220例BV患者和非BV患者的阴道拭子进行了广泛的16 S rRNA基因PCR和焦磷酸测序。根据Amsel临床标准评估BV，并通过革兰氏染色证实。使用系统发育放置工具进行分类学分类，该工具将99%的查询序列读数分配到物种水平。BV妇女阴道细菌群落异质性，通常不是由一个单一的taxon. In BV的情况下，阴道细菌群落占主导地位的卷曲乳杆菌或乳酸杆菌iners。Leptotrichia pastonii和Eggerthella sp.是仅有的两种BV相关细菌（BVAB）与四个Amsel标准中的每一个显著相关。共现分析显示存在几个BVAB亚组，表明存在代谢共依赖性。在没有BV的黑人妇女中观察到更多的几种BVAB。人类阴道细菌生物群是异质性的，其特征是BV女性的物种丰富度和多样性更高;没有物种普遍存在。不同的细菌种类与四种临床标准有不同的关联，这可能是Amsel和Nugent（革兰氏染色）诊断标准之间经常观察到的差异的原因。当按BV状态在单独的组中进行分析时，几种BVAB表现出种族依赖性患病率，这可能导致黑人女性BV发生率增加。在这个项目中开发的工具可以用来研究微生物生态学在不同的设置在高分辨率。
Bacterial vaginosis (BV) is a common condition that is associated with numerous adverse health outcomes and is characterized by poorly understood changes in the vaginal microbiota. We sought to describe the composition and diversity of the vaginal bacterial biota in women with BV using deep sequencing of the 16S rRNA gene coupled with species-level taxonomic identification. We investigated the associations between the presence of individual bacterial species and clinical diagnostic characteristics of BV. Broad-range 16S rRNA gene PCR and pyrosequencing were performed on vaginal swabs from 220 women with and without BV. BV was assessed by Amsel’s clinical criteria and confirmed by Gram stain. Taxonomic classification was performed using phylogenetic placement tools that assigned 99% of query sequence reads to the species level. Women with BV had heterogeneous vaginal bacterial communities that were usually not dominated by a single taxon. In the absence of BV, vaginal bacterial communities were dominated by either Lactobacillus crispatus or Lactobacillus iners. Leptotrichia amnionii and Eggerthella sp. were the only two BV-associated bacteria (BVABs) significantly associated with each of the four Amsel’s criteria. Co-occurrence analysis revealed the presence of several sub-groups of BVABs suggesting metabolic co-dependencies. Greater abundance of several BVABs was observed in Black women without BV. The human vaginal bacterial biota is heterogeneous and marked by greater species richness and diversity in women with BV; no species is universally present. Different bacterial species have different associations with the four clinical criteria, which may account for discrepancies often observed between Amsel and Nugent (Gram stain) diagnostic criteria. Several BVABs exhibited race-dependent prevalence when analyzed in separate groups by BV status which may contribute to increased incidence of BV in Black women. Tools developed in this project can be used to study microbial ecology in diverse settings at high resolution.
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影响因子：
2.1
作者：
Atassi, Fabrice;Servin, Alain L.
通讯作者：
Servin, Alain L.
影响因子：
11.8
作者：
Haggerty, CL;Hillier, SL;Ness, RB
通讯作者：
Ness, RB
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
158.5
作者：
Hillier, SL;Nugent, RP;Klebanoff, MA
通讯作者：
Klebanoff, MA