A macrophage subpopulation promotes airineme-mediated intercellular communication in a matrix metalloproteinase-9 dependent manner.
A macrophage subpopulation promotes airineme-mediated intercellular communication in a matrix metalloproteinase-9 dependent manner.
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巨噬细胞亚群以基质金属蛋白酶-9依赖的方式促进航空素介导的细胞间通讯。
DOI：
10.1016/j.celrep.2023.112818
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发表时间：
2023-07-25
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
组织驻留巨噬细胞是异质的，并执行位置依赖性功能。皮肤巨噬细胞通过介导称为airinemes的细胞突起在长距离细胞间信号传导中发挥有趣的作用。这些巨噬细胞在色素细胞之间传递含有气丝囊泡的信号分子，它们的缺失破坏了气丝介导的信号传导和色素模式的形成。尚不清楚是否相同的巨噬细胞控制这些信号和典型的免疫功能，或者是否有单独的亚群在细胞间通讯中发挥作用。通过高分辨率成像和基因消融方法，我们确定了一个负责airineme介导的信号转导的巨噬细胞亚群。这些似乎是不同于传统的皮肤居民巨噬细胞的阿米巴样形态和更快或扩张的迁移行为。它们类似于外胚层衍生的巨噬细胞，称为变形细胞。嗜酸性粒细胞消融显著降低了空气丝延伸和信号传导。此外，这些阿米巴样细胞/变形细胞需要基质金属蛋白酶-9用于其迁移和空气丝介导的信号传导。这些结果揭示了一个巨噬细胞亚群与专门的功能，在airineme介导的信号，这可能在其他方面的细胞间通讯中发挥作用。TRM是高度异质性的，并且通常执行非免疫功能。Bowman等人发现，一种上皮来源的巨噬细胞亚群，其负责airinemes，一种在斑马鱼色素模式形成中细胞间信号传导所需的长而薄的细胞突起，并且它们在airineme-mediated信号传导中的作用取决于MMP 9活性。
Tissue-resident macrophages are heterogeneous and perform location-dependent functions. Skin resident macrophages play intriguing roles in long-distance intercellular signaling by mediating cellular protrusions called airinemes in zebrafish. These macrophages relay signaling molecules containing airineme vesicles between pigment cells, and their absence disrupts airineme-mediated signaling and pigment pattern formation. It is unknown if the same macrophages control both these signaling and typical immune functions or if a separate subpopulation functions in intercellular communication. With high-resolution imaging and genetic ablation approaches, we identify a macrophage subpopulation responsible for airineme-mediated signaling. These seem to be distinct from conventional skin-resident macrophages by their ameboid morphology and faster or expansive migratory behaviors. They resemble ectoderm-derived macrophages termed metaphocytes. Metaphocyte ablation markedly decreases airineme extension and signaling. In addition, these ameboid/metaphocytes require matrix metalloproteinase-9 for their migration and airineme-mediated signaling. These results reveal a macrophage subpopulation with specialized functions in airineme-mediated signaling, which may play roles in other aspects of intercellular communication. TRMs are highly heterogeneous and often perform non-immune functions. Bowman et al. discover that metaphocytes, an epithelial-derived macrophage subpopulation, are responsible for airinemes, a long, thin cellular protrusion required for intercellular signaling in zebrafish pigment pattern formation, and their role in airineme-mediated signaling depends on MMP9 activity.
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