Distinct compartments of the proepicardial organ give rise to coronary vascular endothelial cells.
Distinct compartments of the proepicardial organ give rise to coronary vascular endothelial cells.
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DOI：
10.1016/j.devcel.2012.01.012
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发表时间：
2012-03-13
影响因子：
11.8
通讯作者：
Tabin, Clifford J.
中科院分区：
文献类型：
--
作者：
Katz, Tamar C.;Singh, Manvendra K.;Degenhardt, Karl;Rivera-Feliciano, Jose;Johnson, Randy L.;Epstein, Jonathan A.;Tabin, Clifford J.
心外膜器官是一种重要的瞬时结构，为各种心脏谱系提供细胞。然而，它对冠状动脉内皮的贡献一直存在争议，在小鸡和小鼠中出现的数据相互矛盾。在这里，我们通过识别两个心外膜标记物 Scleraxis (Scx) 和 Semaphorin3D (Sema3D) 来解决这一冲突，它们在基因上描绘了迄今为止未表征的心外膜亚区室。与先前命运定位的Tbx18/WT-1表达细胞产生血管平滑肌相反，表达Scx和Sema3D的心外膜细胞在体内和体外均产生冠状血管内皮。此外，Sema3D+和Scx+心外膜细胞分别对早期静脉窦和心内膜做出贡献，这两种组织与后期血管内皮形成相关。总而言之，我们的研究表明，心外膜器官是一种分子区室结构，协调了先前的鸡和小鼠数据，并提供了对建立冠状血管内皮的祖细胞群的更全面的了解。
The proepicardial organ is an important transient structure that contributes cells to various cardiac lineages. However, its contribution to the coronary endothelium has been disputed, with conflicting data arising in chick and mouse. Here we resolve this conflict by identifying two proepicardial markers, Scleraxis (Scx) and Semaphorin3D (Sema3D), that genetically delineate heretofore uncharacterized proepicardial subcompartments. In contrast to previously fate mapped Tbx18/WT-1-expressing cells that give rise to vascular smooth muscle, Scx and Sema3D-expressing proepicardial cells give rise to coronary vascular endothelium both in vivo and in vitro. Furthermore, Sema3D+ and Scx+ proepicardial cells contribute to the early sinus venosus and cardiac endocardium, respectively, two tissues linked to vascular endothelial formation at later stages. Taken together, our studies demonstrate that the proepicardial organ is a molecularly compartmentalized structure, reconciling prior chick and mouse data and providing a more complete understanding of the progenitor populations that establish the coronary vascular endothelium.
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