Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production.
Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production.
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人尿液干细胞分化为具有屏障功能和一氧化氮生成的内皮细胞
DOI：
10.1002/sctm.18-0040
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发表时间：
2018-09
影响因子：
6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Liu G;Wu R;Yang B;Deng C;Lu X;Walker SJ;Ma PX;Mou S;Atala A;Zhang Y
内皮细胞（EC）在再生组织的血管重建中起着关键作用。当EC不可用时，干细胞通常用作替代细胞来源。已经使用几种细胞类型来产生EC，例如脐带血管，或从体干细胞、胚胎或诱导多能干细胞分化。然而，后者具有慢性免疫排斥和肿瘤发生的潜在风险。自体内皮前体是一种理想的资源，但目前需要侵入性手术从患者自己的血管或骨髓中获得它们。因此，本研究的目的是确定尿源性干细胞（USCs）是否可以在体外分化为功能性EC。然后使用内皮分化培养基将尿源性细胞分化为内皮谱系细胞14天。在体外诱导的USCs中评估形态学和超微结构的变化以及功能性内皮标志物表达。然后将分化的USCs移植物皮下注射到裸鼠中。与未分化的USCs相比，诱导的USCs在体外和体内表达显著更高水平的EC特异性标志物（CD 31，vWF，eNOS）。此外，分化的USC形成了复杂的管状网络，并呈现出与对照组相似的紧密连接、迁移和侵袭能力以及产生一氧化氮（NO）的能力。使用USCs作为血管的自体EC来源，组织工程策略可以通过适合快速临床转化的无创、简单和低成本方法产生足够数量的细胞。干细胞转化医学2018干细胞转化医学2018;7：686-698
Endothelial cells (ECs) play a key role in revascularization within regenerating tissue. Stem cells are often used as an alternative cell source when ECs are not available. Several cell types have been used to give rise to ECs, such as umbilical cord vessels, or differentiated from somatic stem cells, embryonic, or induced pluripotent stem cells. However, the latter carry the potential risk of chronic immune rejection and oncogenesis. Autologous endothelial precursors are an ideal resource, but currently require an invasive procedure to obtain them from the patient's own blood vessels or bone marrow. Thus, the goal of this study was to determine whether urine‐derived stem cells (USCs) could differentiate into functional ECs in vitro. Urine‐derived cells were then differentiated into cells of the endothelial lineage using endothelial differentiation medium for 14 days. Changes in morphology and ultrastructure, and functional endothelial marker expression were assessed in the induced USCs in vitro. Grafts of the differentiated USCs were then subcutaneously injected into nude mice. Induced USCs expressed significantly higher levels of specific markers of ECs (CD31, vWF, eNOS) in vitro and in vivo, compared to nondifferentiated USCs. In addition, the differentiated USC formed intricate tubular networks and presented similar tight junctions, and migration and invasion ability, as well as ability to produce nitric oxide (NO) compared to controls. Using USCs as autologous EC sources for vessel, tissue engineering strategies can yield a sufficient number of cells via a noninvasive, simple, and low‐cost method suitable for rapid clinical translation. stem cells translational medicine 2018 Stem Cells Translational Medicine 2018;7:686–698
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影响因子：
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作者：
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