Self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours.
Self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours.
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DOI：
10.1371/journal.pone.0053980
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Lang R;Liu G;Shi Y;Bharadwaj S;Leng X;Zhou X;Liu H;Atala A;Zhang Y
尽管保存器官、组织和分离的细胞的方法是成功的，但在储存用于细胞分布和运输的过程中维持体液中的干细胞活力和功能仍然未被探索。本研究的目的是表征尿液标本最佳保存长达24小时后的尿源性干细胞（USCs）。共收集了12名健康男性（年龄范围20-54岁）的415份尿液标本。24小时内约有6×104细胞从泌尿系统脱落。24小时后，从储存的尿液标本中获得至少100个USC克隆，并保持与新鲜USCs相似的生物学特性。原代培养的USCs呈米粒状，表达间充质干细胞表面标志，端粒酶活性高，核型正常。重要的是，保存的细胞保留了双能分化能力。分化的USCs在肌源性分化培养基中表达肌源性特异性蛋白和收缩功能，在尿路上皮分化培养基中表达尿路上皮细胞特异性标志物和屏障功能。这些数据表明，高达75%的新鲜USCs可以安全地在尿液中保存24小时，并且这些储存在尿液中的细胞保留了其原始干细胞特性，表明保存的USCs可用于基于细胞的治疗或临床诊断。
Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20–54 years old). About 6×104 cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a “rice grain” shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis.
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影响因子：
3.7
作者：
Jiang S;Bailey AS;Goldman DC;Swain JR;Wong MH;Streeter PR;Fleming WH
通讯作者：
Fleming WH
影响因子：
4.1
作者：
Bharadwaj, Shantaram;Liu, Guihua;Zhang, Yuanyuan
通讯作者：
Zhang, Yuanyuan
影响因子：
14
作者：
Bodin, Aase;Bharadwaj, Shantaram;Zhang, Yuanyuan
通讯作者：
Zhang, Yuanyuan
DOI：
10.1016/s0301-2115(02)00465-7
发表时间：
2003-06-10
影响因子：
2.6
作者：
Isachenko, E;Isachenko, V;Nawroth, F
通讯作者：
Nawroth, F
影响因子：
0.9
作者：
Mühlbacher, F;Langer, F;Mittermayer, C
通讯作者：
Mittermayer, C