Loss of rice PARAQUAT TOLERANCE 3 confers enhanced resistance to abiotic stresses and increases grain yield in field
Loss of rice PARAQUAT TOLERANCE 3 confers enhanced resistance to abiotic stresses and increases grain yield in field
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水稻失去百草枯耐受性 3 可增强对非生物胁迫的抵抗力并提高田间谷物产量
DOI：
10.1101/2020.02.22.961151
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Xiang Cheng-Bin
中科院分区：
文献类型：
--
作者：
Alfatih Alamin;Wu Jie;Jan Sami Ullah;Zhang Zi-Sheng;Xia Jin-Qiu;Xiang Cheng-Bin
植物在自然界中经常遭受环境胁迫，并进化出复杂而有效的机制来应对这些胁迫。为了在生长和胁迫反应之间取得平衡，植物配备了有效的手段，在胁迫减弱时关闭激活的胁迫反应。我们先前揭示了拟南芥PQT3的这种开关机制，它的敲除显著增强了对非生物胁迫的抗性。为了探索水稻同源基因OsPQT3是否在功能上保守，我们用CRISPR-Cas9技术产生了三个基因敲除突变体。OsPQT3基因敲除突变体(OSPqt3)表现出对氧化和盐胁迫的抗性增强，OsGPX1、OsAPX1和OsSOD1的表达增加。更重要的是，无论是在温室盐胁迫下还是在田间条件下，SPQt3突变体的农艺表现都比野生型显著提高，产量也高于野生型。我们进一步表明，OsPQT3与AtPQT3一样，在应对氧化和其他非生物胁迫时迅速下调。综上所述，这些结果支持我们之前的发现，即AtPQT3在胁迫反应中起着关闭开关的作用，这在水稻中是很保守的。因此，PQT3基因座为利用基因编辑技术提高作物抗逆性提供了一个很有前景的候选基因。
Plants frequently suffer from environmental stresses in nature and have evolved sophisticated and efficient mechanisms to cope with the stresses. To balance between growth and stress response, plants are equipped with efficient means to switch off the activated stress responses when stresses diminish. We previously revealed such an off-switch mechanism conferred by Arabidopsis PQT3, knockout of which significantly enhances resistance to abiotic stresses. To explore whether the rice homolog OsPQT3 is functionally conserved, we generated three knockout mutants with CRISPR-Cas9 technology. The OsPQT3 knockout mutants (ospqt3) display enhanced resistance to oxidative and salt stress with elevated expression of OsGPX1, OsAPX1, and OsSOD1. More importantly, the ospqt3 mutants show significantly enhanced agronomic performance with higher yield compared with the wild type under salt stress in greenhouse as well as in field conditions. We further showed that OsPQT3 was rapidly down regulated in response to oxidative and other abiotic stresses as AtPQT3. Taken together, these results support our previous findings that AtPQT3 acts as an off-switch in stress response, which is well conserved in rice. Therefore, PQT3 locus provides a promising candidate for crop improvement with enhanced stress resistance by gene editing technology.
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DOI：
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发表时间：
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DOI：
10.1199/tab.0131
发表时间：
2010-01-01
期刊：
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影响因子：
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作者：
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