Long noncoding RNA associated-competing endogenous RNAs in gastric cancer.
Long noncoding RNA associated-competing endogenous RNAs in gastric cancer.
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胃癌中长非编码RNA相关竞争性内源RNA
DOI：
10.1038/srep06088
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发表时间：
2014-08-15
影响因子：
4.6
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Xia T;Liao Q;Jiang X;Shao Y;Xiao B;Xi Y;Guo J
一些长非编码RNAs(LncRNAs)作为竞争内源RNAs(CeRNAs)在基因表达调控中发挥重要作用。然而，LncRNA相关的ceRNAs在肿瘤发生中的作用还不完全清楚。在此，我们基于胃癌的lncRNA微阵列数据、生物信息学算法miRcode和microRNA(MiRNA)靶标数据库TarBase，首次构建了一个lncRNA-miRNA-mRNA网络。然后，我们通过头颈部鳞状细胞癌、前列腺癌、乳头状甲状腺癌、垂体促性腺激素瘤、卵巢癌和慢性淋巴细胞白血病等六种其他癌症的数据证实了这一点。结果显示了一个明显的与癌症相关的Cerna网络。涉及8个lncRNAs(AC009499.1、GACAT1、GACAT3、H19、LINC00152、AP000288.2、FER1L4和Rp4-620F22.3)和9个miRNAs(miR-18a-5p、miR-18b-5p、miR-19a-3p、miR-20b-5p、miR-106a-5p、miR-106b-5p、miR-31-5p、miR-139-5p和miR-195-5p)。例如，通过其miRNA反应元件(MRE)竞争miR-106a-5p，lncRNA-FER1L4调控PTEN、RB1、RUNX1、VEGFA、CDKN1A、E2F1、HIPK3、IL-10和PAK7的表达。此外，细胞实验结果表明，FER1L4-小干扰RNA(SiRNA)同时抑制FER1L4和RB1的mRNA水平。这些结果表明，lncRNAs含有MRE，并在癌症的转录后调控中发挥重要作用。
Some long noncoding RNAs (lncRNAs) play important roles in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the roles of lncRNA associated-ceRNAs in oncogenesis are not fully understood. Here, based on lncRNA microarray data of gastric cancer, bioinformatic algorithm miRcode and microRNA (miRNA) targets database TarBase, we first constructed an lncRNA-miRNA-mRNA network. Then, we confirmed it by data of six types of other cancer including head and neck squamous cell carcinoma, prostate cancer, papillary thyroid carcinoma, pituitary gonadotrope tumors, ovarian cancer and chronic lymphocytic leukemia. The results showed a clear cancer-associated ceRNA network. Eight lncRNAs (AC009499.1, GACAT1, GACAT3, H19, LINC00152, AP000288.2, FER1L4 and RP4-620F22.3) and nine miRNAs (miR-18a-5p, miR-18b-5p, miR-19a-3p, miR-20b-5p, miR-106a-5p, miR-106b-5p, miR-31-5p, miR-139-5p and miR-195-5p) were involved. For instance, through its miRNA response elements (MREs) to compete for miR-106a-5p, lncRNA-FER1L4 regulates the expression of PTEN, RB1, RUNX1, VEGFA, CDKN1A, E2F1, HIPK3, IL-10 and PAK7. Furthermore, cellular experimental results indicated that FER1L4-small interfering RNA (siRNA) simultaneously suppressed FER1L4 and RB1 mRNA level. These results suggest that lncRNAs harbor MREs and play important roles in post-transcriptional regulation in cancer.
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影响因子：
3.7
作者：
Khachane AN;Harrison PM
通讯作者：
Harrison PM
starBase v2.0：从大规模 CLIP-Seq 数据中解码 miRNA-ceRNA、miRNA-ncRNA 和蛋白质-RNA 相互作用网络
影响因子：
14.9
作者：
Li JH;Liu S;Zhou H;Qu LH;Yang JH
通讯作者：
Yang JH
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
在致癌 BRAF 诱导的黑色素瘤小鼠模型中体内鉴定肿瘤抑制性 PTEN ceRNA。
影响因子：
64.5
作者：
Karreth FA;Tay Y;Perna D;Ala U;Tan SM;Rust AG;DeNicola G;Webster KA;Weiss D;Perez-Mancera PA;Krauthammer M;Halaban R;Provero P;Adams DJ;Tuveson DA;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
28.2
作者：
Karreth FA;Pandolfi PP
通讯作者：
Pandolfi PP