Dispersion characteristics of oral microbial communities in a built environment

Dispersion characteristics of oral microbial communities in a built environment
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建筑环境中口腔微生物群落的扩散特征

DOI:
10.1002/2475-8876.12261
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发表时间:
2022
影响因子:
0.9
通讯作者:
Hayama Hirofumi
Hayama Hirofumi
中科院分区:
--
文献类型:
--
作者:
Yanagi U;Kato Shinsuke;Nagano Hideaki;Ito Kazuhide;Yamanaka Toshio;Momoi Yoshihisa;Kobayashi Hikaru;Hayama Hirofumi

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自2005年以来,下一代测序(NGS)技术的进步已经彻底改变了生物科学。NGS技术的一个特殊应用是阐明建筑环境中的微生物组。目前,我们正在进行一系列基于环境微生物组动态测量的群体感染机制的阐明和控制研究。本研究的目的是阐明口腔细菌在建筑环境中的分散特性。在日本七所大学中,对居住者的手和口腔、门把手、桌子和键盘表面以及实验室空气中的细菌群落进行了调查。厚壁菌门、变形菌门、放线菌门、拟杆菌门和梭杆菌门的相对丰度中位数分别为41%、31%、12%、7%和3%。检出的主要菌属为链球菌属(27.6%)、嗜血杆菌属(7.0%)、葡萄球菌属(5.6%)、奈瑟菌属(5.6%)、棒状杆菌属(4.7%)、罗氏菌属(3.2%)、普雷沃菌属(3.0%)、梭杆菌属(2.6%)、韦荣氏球菌属(1.7%)、纤毛菌属(1.7%)、增水杆菌属(1.7%)、乳杆菌属(1.3%)、不动杆菌属(1.3%)和放线菌属(1.1%)。口腔细菌包括放线菌、棒状杆菌、梭杆菌、嗜血杆菌、纤毛菌、奈瑟菌、普雷沃菌、罗氏菌和链球菌。此外,在所有7所大学的所有样品中,包括手和口腔、门把手、桌子和PC键盘表面以及实验室空气中,都观察到了产黑色素普雷沃氏菌和胶质罗氏菌。
Advances in next‐generation sequencing (NGS) technologies since 2005 have revolutionized biological science. One particular application of NGS technologies is to elucidate microbiomes in built environments. We are currently conducting a series of studies on the elucidation and control of mass infection mechanisms based on dynamic measurement of environment microbiomes. The objective of this study is to clarify the dispersion characteristics of oral bacteria in the built environment. Bacterial communities from occupants’ hands and oral cavities, doorknobs, desk and keyboard surfaces, and air in laboratories were investigated in seven Japanese universities. The median relative abundances of Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were 41%, 31%, 12%, 7%, and 3%, respectively. Moreover, the main genera detected wereStreptococcus(27.6%),Haemophilus(7.0%),Staphylococcus(5.6%),Neisseria(5.6%),Corynebacterium(4.7%),Rothia(3.2%),Prevotella(3.0%),Fusobacterium(2.6%),Veillonella(1.7%),Leptotrichia(1.7%),Enhydrobacter(1.7%),Lactobacillus(1.3%),Acinetobacter(1.3%), andActinomyces(1.1%). The oral bacteriaActinomyces,Corynebacterium,Fusobacterium,Haemophilus,Leptotrichia,Neisseria,Prevotella,Rothia, andStreptococcuswere observed in indoor air and on surfaces as well as in oral cavities. Furthermore,Prevotella melaninogenicaandRothia mucilaginosawere observed in all samples, including those from hands and oral cavities, doorknobs, desk and PC keyboard surfaces, and air in laboratories, in all seven universities.
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DOI: --
发表时间: 2019
期刊: Comprehensive Biotechnology
影响因子: --
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