Bacterial diversity among four healthcare-associated institutes in Taiwan.
Bacterial diversity among four healthcare-associated institutes in Taiwan.
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DOI：
10.1038/s41598-017-08679-3
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发表时间：
2017-08-15
影响因子：
4.6
通讯作者：
Liou ML
中科院分区：
文献类型：
--
作者：
Chen CH;Lin YL;Chen KH;Chen WP;Chen ZF;Kuo HY;Hung HF;Tang CY;Liou ML
室内微生物群落对人类健康具有重要意义，特别是在卫生保健机构（HCI）中。决定建筑环境中微生物群落多样性和组成的因素仍不清楚。在此，我们使用16 S rRNA扩增子测序，以调查之间的关系，建筑属性和表面细菌群落之间的四个HCI位于三个建筑物。我们研究了表面细菌群落和环境参数的建筑物提供了不同的通风类型和比较结果使用狄利克雷多项式混合（DMM）为基础的方法。共分析了来自4种HCI的203份样本。使用基于DMM的方法对四种细菌群落进行分组，这些细菌群落与4种HCI中的细菌群落高度相似。自然通风建筑与空调通风建筑的α-多样性和β-多样性不同。每个建筑物的细菌来源组成各不相同。发现9个属是所有地区共有的核心微生物群，其中不动杆菌属、肠杆菌属、假单胞菌属和葡萄球菌属被认为是医疗保健相关病原体（HAP）。观察到的环境参数之间的关系，如核心微生物群和表面细菌多样性表明，我们可以通过创建新的卫生协议，调整通风设计，并进一步了解HAP的传播途径来管理室内环境。
Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs.
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影响因子：
4.6
作者：
Ferrari B;Winsley T;Ji M;Neilan B
通讯作者：
Neilan B
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.2
作者：
Núñez, S;Moreno, A;Villar, J
通讯作者：
Villar, J
影响因子：
3.7
作者：
Hewitt KM;Mannino FL;Gonzalez A;Chase JH;Caporaso JG;Knight R;Kelley ST
通讯作者：
Kelley ST
影响因子：
3.7
作者：
Flores GE;Bates ST;Knights D;Lauber CL;Stombaugh J;Knight R;Fierer N
通讯作者：
Fierer N