Characterization of cell surface polypeptides of unfertilized, fertilized, and protease-treated zona-free mouse eggs.
Characterization of cell surface polypeptides of unfertilized, fertilized, and protease-treated zona-free mouse eggs.
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未受精、受精和蛋白酶处理的无透明带小鼠卵细胞表面多肽的表征。
DOI：
10.1002/mrd.1120230109
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Howe,AM
中科院分区：
文献类型：
--
作者：
Boldt,J;Gunter,LE;Howe,AM
The polypeptide composition of unfertilized, fertilized, and protease-treated zona-free mouse eggs was evaluated in this study. Zona-free eggs were radioiodinated by an Iodogen-catalyzed reaction. Light microscopic autoradiography of egg sections revealed that labeling was restricted to the cell surface. Labeled eggs were solubilized, and cell surface polypeptides were identified by one-dimensional SDS polyacrylamide gel electro-phoresis and autoradiography. The unfertilized egg demonstrated 8–10 peptides that incorporated 125 I, with major bands observed at approximately 145–150, 94, and 23 kilo-daltons (kD). Zona-free eggs fertilized in vitro and then radiolabeled demonstrated several new bands in comparison to unfertilized eggs, with a major band appearing at approxi mately 36 kD. Treatment of radiolabeled unfertilized eggs with either trypsin or chymo-trypsin (1 mg/ml for 5–20 min) caused enzyme-specific modifications in labeled polypep tides. Trypsin (T) treatment resulted in time-dependant modification of the three major peptides at 145–150, 94, and 23 kD. Chymotrypsin (CT) treatment, in contrast, was asso ciated with loss or modification of the 94 kD band, with no apparent effect on either the 145–150 or 23 kD band. Taken together with previous data indicating that T or CT egg treatment interferes with sperm-egg attachment and fusion (Boldt et al.: Biol Reprod 39: 19–27, 1988), these results suggest a possible role for the 94 kD protein in sperm-egg interaction.
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DOI：
10.1002/mrd.1120130304
发表时间：
1986
期刊：
Gamete Research
影响因子：
--
作者：
J. Boldt;D. Wolf
通讯作者：
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影响因子：
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作者：
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DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
Y. Hirao;R. Yanagimachi
通讯作者：
R. Yanagimachi
酶促改变小鼠卵质膜与精子相互作用的能力。
影响因子：
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作者：
Boldt,J;Howe,AM;Preble,J
通讯作者：
Preble,J
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影响因子：
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作者：
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