Aflatoxin B₁ degradation by a Pseudomonas strain.
Aflatoxin B₁ degradation by a Pseudomonas strain.
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假单胞菌菌株对黄曲霉毒素 B(1) 的降解。
DOI：
10.3390/toxins6103028
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发表时间：
2014-10-23
期刊：
影响因子：
4.2
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Sangare L;Zhao Y;Folly YM;Chang J;Li J;Selvaraj JN;Xing F;Zhou L;Wang Y;Liu Y
黄曲霉毒素B1 （AFB1）是最有效的天然诱变剂和致癌物之一，对食品工业和动物生产造成重大威胁。本研究从籽粒和土壤中收集了25株具有AFB1还原活性的分离菌。根据其降解效果，选择分离物N17-1进行进一步鉴定，鉴定为铜绿假单胞菌（Pseudomonas aeruginosa）。P. aeruginosa N17-1在NB培养基中37℃孵育72 h后，对AFB1、AFB2和AFM1的降解率分别为82.8%、46.8%和31.9%。分离物N17-1的培养上清液能有效降解AFB1，而活细胞和细胞内提取物的降解效果较差。研究了影响AFB1被培养上清降解的因素。在55°C时观察到最大的降解。Mn2+和Cu2+离子是降解AFB1的活化剂，而Mg2+、Li+、Zn2+、Se2+、Fe3+离子是强抑制剂。蛋白酶K和蛋白酶K + SDS处理显著降低了培养上清液的降解活性。初步LC-QTOF/MS分析未发现降解产物，说明AFB1被代谢为与AFB1化学性质不同的降解产物。结果表明，P. aeruginosa N17-1对AFB1的降解是酶促的，具有很大的工业应用潜力。这是首次报道铜绿假单胞菌具有降解黄曲霉毒素的能力。
Aflatoxin B1 (AFB1), one of the most potent naturally occurring mutagens and carcinogens, causes significant threats to the food industry and animal production. In this study, 25 bacteria isolates were collected from grain kernels and soils displaying AFB1 reduction activity. Based on its degradation effectiveness, isolate N17-1 was selected for further characterization and identified as Pseudomonas aeruginosa. P. aeruginosa N17-1 could degrade AFB1, AFB2 and AFM1 by 82.8%, 46.8% and 31.9% after incubation in Nutrient Broth (NB) medium at 37 °C for 72 h, respectively. The culture supernatant of isolate N17-1 degraded AFB1 effectively, whereas the viable cells and intra cell extracts were far less effective. Factors influencing AFB1 degradation by the culture supernatant were investigated. Maximum degradation was observed at 55 °C. Ions Mn2+ and Cu2+ were activators for AFB1 degradation, however, ions Mg2+, Li+, Zn2+, Se2+, Fe3+ were strong inhibitors. Treatments with proteinase K and proteinase K plus SDS significantly reduced the degradation activity of the culture supernatant. No degradation products were observed based on preliminary LC-QTOF/MS analysis, indicating AFB1 was metabolized to degradation products with chemical properties different from that of AFB1. The results indicated that the degradation of AFB1 by P. aeruginosa N17-1 was enzymatic and could have a great potential in industrial applications. This is the first report indicating that the isolate of P. aeruginosa possesses the ability to degrade aflatoxin.
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DOI：
10.1080/03601234.2013.836868
发表时间：
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影响因子：
2
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影响因子：
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影响因子：
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