Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine.
Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine.
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DOI：
10.1186/gb-2011-12-11-r116
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发表时间：
2011-11-23
期刊：
影响因子：
12.3
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Zheng P;Xia Y;Xiao G;Xiong C;Hu X;Zhang S;Zheng H;Huang Y;Zhou Y;Wang S;Zhao GP;Liu X;St Leger RJ;Wang C
子囊菌科真菌虫草属中的种被认为是绿僵菌属的有性型。后者已被广泛用作昆虫生物防治剂。冬虫夏草在传统中药中的应用价值很高，但负责生物活性成分生物合成、昆虫致病性以及控制性和结果的基因尚未确定。在这里，我们报告的模式种蛹虫草的基因组序列。系统基因组学分析表明，在冬虫夏草/绿僵菌属的不同物种已经演变成昆虫病原体彼此独立，它们相似的大分泌和基因家族的扩展是由于趋同进化。然而，相对于其他真菌，包括绿僵菌属，许多蛋白质家族在C. militaris，这表明一个更受限制的生态。与其作为药物安全使用的长期记录一致，虫草基因组不包含已知人类真菌毒素的基因。我们建立了C。北五味子是有性异宗配合的，但是非常不寻常的是，在没有相反的交配型伴侣的情况下也能结果实。转录谱分析表明，出菇涉及Zn 2Cys 6型转录因子和MAPK途径的诱导;然而，与其他真菌不同的是，PKA途径未被激活。这些数据提供了对冬虫夏草生物学的更好理解，并将有助于开发由真菌产生的药用化合物。
Species in the ascomycete fungal genus Cordyceps have been proposed to be the teleomorphs of Metarhizium species. The latter have been widely used as insect biocontrol agents. Cordyceps species are highly prized for use in traditional Chinese medicines, but the genes responsible for biosynthesis of bioactive components, insect pathogenicity and the control of sexuality and fruiting have not been determined. Here, we report the genome sequence of the type species Cordyceps militaris. Phylogenomic analysis suggests that different species in the Cordyceps/Metarhizium genera have evolved into insect pathogens independently of each other, and that their similar large secretomes and gene family expansions are due to convergent evolution. However, relative to other fungi, including Metarhizium spp., many protein families are reduced in C. militaris, which suggests a more restricted ecology. Consistent with its long track record of safe usage as a medicine, the Cordyceps genome does not contain genes for known human mycotoxins. We establish that C. militaris is sexually heterothallic but, very unusually, fruiting can occur without an opposite mating-type partner. Transcriptional profiling indicates that fruiting involves induction of the Zn2Cys6-type transcription factors and MAPK pathway; unlike other fungi, however, the PKA pathway is not activated. The data offer a better understanding of Cordyceps biology and will facilitate the exploitation of medicinal compounds produced by the fungus.
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影响因子：
4.5
作者：
Gao Q;Jin K;Ying SH;Zhang Y;Xiao G;Shang Y;Duan Z;Hu X;Xie XQ;Zhou G;Peng G;Luo Z;Huang W;Wang B;Fang W;Wang S;Zhong Y;Ma LJ;St Leger RJ;Zhao GP;Pei Y;Feng MG;Xia Y;Wang C
通讯作者：
Wang C
影响因子：
5.1
作者：
Duan, Zhibing;Shang, Yianfang;Wang, Chengshu
通讯作者：
Wang, Chengshu
DOI：
10.1073/pnas.132212899
发表时间：
2002-06-25
影响因子：
11.1
作者：
Freitag, M;Williams, RL;Selker, EU
通讯作者：
Selker, EU
影响因子：
4.2
作者：
de Faria, Marcos R.;Wraight, Stephen P.
通讯作者：
Wraight, Stephen P.
影响因子：
3
作者：
Brown, DW;McCormick, SP;Desjardins, AE
通讯作者：
Desjardins, AE