Quantitation of alternative splicing variants of lactate dehydrogenase C gene in testes of adult yak, sexually immature yak calf and sterile male hybrid of yak
Quantitation of alternative splicing variants of lactate dehydrogenase C gene in testes of adult yak, sexually immature yak calf and sterile male hybrid of yak
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成年牦牛、性成熟牦牛犊及不育雄杂种牦牛睾丸中乳酸脱氢酶C基因选择性剪接变异体的定量
DOI：
10.4141/cjas2012-018
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发表时间：
2012-09
影响因子：
1
通讯作者：
郑玉才
中科院分区：
文献类型：
--
作者：
黄林;金素钰;徐亚欧;李玉萍;林亚秋;郑玉才
黄磊，金世英，金绍英。， Xu, Y-O。Li, Y-P。林，Y-Q。郑，Y-C。2012. 成年牦牛、性成熟犊牛和不育杂交牦牛睾丸乳酸脱氢酶C基因选择性剪接变异体的定量分析。可以。j .似的。科学学报，42(2):291-296。本研究的主要目的是定量分析牦牛（Bos grunniens）和雄性不育杂交牦牛睾丸中乳酸脱氢酶C （ldhc）基因的选择性剪接。睾丸中ldhc cDNA的RT-PCR扩增显示，mRNA转录物中一个或多个外显子的缺失形成了8个剪接变异体。缺失的外显子主要位于外显子7和4。外显子的缺失导致部分变异的读框移位和停止密码子的形成。利用ldhc变异特异性引物进行实时定量RT-PCR分析发现，性成熟牦牛犊牛（n=6）和雄性不育杂交牛-牦牛（n=4）的睾丸中ldhc全长mRNA水平显著低于正常牦牛。
Huang, L., Jin, S-Y., Xu, Y-O., Li, Y-P., Lin, Y-Q. and Zheng, Y-C. 2012. Quantitation of alternative splicing variants of lactate dehydrogenase C gene in testes of adult yak, sexually immature yak calf and sterile male hybrid of yak. Can. J. Anim. Sci. 92: 291–296. The main objective of the present study was to analyze quantitatively the alternative splicing of the lactate dehydrogenase C (ldhc) gene in the testes of yak (Bos grunniens) and male sterile yak hybrid. RT-PCR amplification of ldhc cDNA in the testes revealed eight splice variants formed by the deletion of one or more exons in the mRNA transcripts. The deleted exons occur mostly in exons 7 and 4. The deletion of exons caused reading frame shift and formation of stop codon in some variants. Quantitative real-time RT-PCR analysis using ldhc variant-specific primers showed that the mRNA level of full length ldhc decreased dramatically in the testes of sexually immature yak calf (n=6) and male sterile hybrid cattle–yak (n=4) compared with that of...
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.6
作者：
Kroft, TL;Jethanandani, P;Goldberg, E
通讯作者：
Goldberg, E
DOI：
10.1002/jez.1401560202
发表时间：
1964-07
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
A. Blanco;W. Zinkham;L. Kupchyk
通讯作者：
A. Blanco;W. Zinkham;L. Kupchyk
影响因子：
--
作者：
Coonrod, Scott;Vitale, Alejandra;Goldberg, Erwin
通讯作者：
Goldberg, Erwin
影响因子：
3.7
作者：
A. Nygård;C. Jørgensen;S. Cirera;M. Fredholm
通讯作者：
A. Nygård;C. Jørgensen;S. Cirera;M. Fredholm