High Index Values of Enzyme-Linked Immunosorbent Assay for BP180 at Baseline Predict Relapse in Patients With Bullous Pemphigoid.
High Index Values of Enzyme-Linked Immunosorbent Assay for BP180 at Baseline Predict Relapse in Patients With Bullous Pemphigoid.
复制标题
DOI：
10.3389/fmed.2018.00139
复制
发表时间：
2018
影响因子：
3.9
通讯作者：
Nakama T
中科院分区：
文献类型：
--
作者：
Koga H;Teye K;Ishii N;Ohata C;Nakama T
大疱性类天疱疮是最常见的自身免疫性大疱性疾病。在免疫学上，BP的特征在于存在循环抗表皮基底膜区（BMZ）抗体。患者抗体靶向的BMZ中的自身抗原主要是BP 180（XVII型胶原）和BP 230。以往的研究表明，针对BP中BP 180的免疫优势区、第16非胶原结构域（NC 16 A）的IgG和抗BP 180 NC 16 A IgE与疾病活动性相关。在细胞因子谱中，IL-6、TNF-α、IL-15和CCL 18的血清水平与疾病的严重程度或活动性相关。血嗜酸性粒细胞增多是常见的，特别是在严重的情况下。这些生物标志物有助于评估治疗效果和疾病严重程度。由于疾病复发的频率很高，在治疗开始时（基线）预测复发必须对临床医生有益。因此，我们评估了基线时复发BP患者（n = 16）和未复发BP患者（n = 31）的生物标志物抗BP 180 IgG（BP 180 ELISA）、抗BP 230 IgG（BP 230 ELISA）、外周嗜酸性粒细胞和血清IgE。我们发现复发组BP 180 ELISA的指数值显著较高，而BP 230 ELISA、外周血嗜酸性粒细胞和血清IgE无显著差异。这项研究表明，基线时BP 180 ELISA的高指数值（临界值，53.09 U/mL;灵敏度，81.3%;特异性，48.4%）可能预测BP患者的复发。这可能有助于临床医生治疗BP患者的决策，关于治疗的持续时间和强度。
Bullous pemphigoid (BP) presenting with erythema plaques and tense blisters is the most frequent autoimmune bullous disease. Immunologically, BP is characterized by the presence of circulating anti-epidermal basement membrane zone (BMZ) antibodies. The autoantigens in BMZs targeted by patient's antibodies are mainly BP180 (type XVII collagen) and BP230. Previous reports have indicated that IgG to the immunodominant region of BP180 in BP, 16th non-collagenous domain (NC16A), and anti-BP180NC16A IgE are related to disease activity. In the cytokine profile, serum levels of IL-6, TNF-α, IL-15, and CCL18 were associated with the severity or activity of the disease. Blood eosinophilia is seen frequently, especially in severe cases. These biomarkers are helpful to evaluate efficacy of treatment and disease severity. Due to the high frequency of disease relapse, prediction of relapse at initiation of treatment (baseline) must be beneficial for clinicians. Therefore, we evaluated biomarkers anti-BP180 IgG (BP180 ELISA), anti-BP230 IgG (BP230 ELISA), peripheral eosinophils, and serum IgE at baseline between BP patients with (n = 16) and without (n = 31) relapse. We found significantly higher index values of BP180 ELISA in the relapse group, whereas no significant difference was found in BP230 ELISA, peripheral eosinophils, and serum IgE. This study indicated that a high index value of BP180 ELISA (cutoff value, 53.09 U/mL; sensitivity, 81.3%; specificity, 48.4%) at baseline may predict relapse in patients with BP. This may help clinicians treating BP patients in decision-making regarding duration and intensity of treatment.
登录
查看更多内容
影响因子：
3.5
作者：
Kasperkiewicz, Michael;Zillikens, Detlef;Schmidt, Enno
通讯作者：
Schmidt, Enno
影响因子：
4.6
作者：
Amo, Y;Ohkawa, T;Hashimoto, T
通讯作者：
Hashimoto, T
影响因子：
10.3
作者：
Bernard, P;Bedane, C;Bonnetblanc, JM
通讯作者：
Bonnetblanc, JM
DOI：
10.1111/jdv.13839
发表时间：
2017-01-01
影响因子：
9.2
作者：
Lee, J. H.;Kim, T. H.;Kim, S. -C.
通讯作者：
Kim, S. -C.
影响因子：
6.5
作者：
WOODLEY, D;SAUDER, D;QWARNSTROM, E
通讯作者：
QWARNSTROM, E