me31B regulates stem cell homeostasis by preventing excess dedifferentiation in the Drosophila male germline.
me31B regulates stem cell homeostasis by preventing excess dedifferentiation in the Drosophila male germline.
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DOI：
10.1242/jcs.258757
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发表时间：
2021-07-15
影响因子：
4
通讯作者：
Yamashita YM
中科院分区：
文献类型：
--
作者：
Jensen L;Venkei ZG;Watase GJ;Bisai B;Pletcher S;Lee CY;Yamashita YM
组织特异性干细胞通过在生物体的整个生命过程中提供持续的分化细胞供应来维持组织稳态。分化/分化细胞可以通过去分化恢复干细胞身份，以帮助维持干细胞库超过个体干细胞的寿命。尽管去分化对于维持干细胞群体是重要的，但据推测它是肿瘤发生的基础。因此，这个过程必须严格控制。在这里，我们表明，翻译调节，me 31 B，在防止果蝇雄性生殖细胞过度去分化中起着至关重要的作用：在me 31 B的情况下，精原细胞去分化成生殖干细胞（GSC）在显着提高的频率。我们的研究结果表明，过度的去分化可能是由于一氧化氮合酶，生殖细胞的身份和GSC维持的关键调节器的失调。总之，我们的数据揭示了去分化的负调控，以平衡干细胞维持与分化。总结：me 31 B被鉴定为果蝇睾丸中精原细胞向生殖系干细胞去分化的负调节因子，其通过下调nos表达的能力。
Tissue-specific stem cells maintain tissue homeostasis by providing a continuous supply of differentiated cells throughout the life of organisms. Differentiated/differentiating cells can revert back to a stem cell identity via dedifferentiation to help maintain the stem cell pool beyond the lifetime of individual stem cells. Although dedifferentiation is important for maintaining the stem cell population, it is speculated that it underlies tumorigenesis. Therefore, this process must be tightly controlled. Here, we show that a translational regulator, me31B, plays a critical role in preventing excess dedifferentiation in the Drosophila male germline: in the absence of me31B, spermatogonia dedifferentiate into germline stem cells (GSCs) at a dramatically elevated frequency. Our results show that the excess dedifferentiation is likely due to misregulation of nos, a key regulator of germ cell identity and GSC maintenance. Taken together, our data reveal negative regulation of dedifferentiation to balance stem cell maintenance with differentiation. Summary: me31B is identified as a negative regulator of dedifferentiation from spermatogonia to germline stem cells in the Drosophila testis via its ability to downregulate nos expression.
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DOI：
10.1261/rna.062208.117
发表时间：
2017-10
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Götze M;Dufourt J;Ihling C;Rammelt C;Pierson S;Sambrani N;Temme C;Sinz A;Simonelig M;Wahle E
通讯作者：
Wahle E
JNK信号传导会触发慢性应激过程中的精子去分化，以维持果蝇睾丸中的种系干细胞库。
影响因子：
7.7
作者：
Herrera SC;Bach EA
通讯作者：
Bach EA
影响因子：
4.6
作者：
Li, Yun;Maines, Jean Z.;Buszczak, Michael
通讯作者：
Buszczak, Michael
影响因子：
10.5
作者：
MCKEARIN, DM;SPRADLING, AC
通讯作者：
SPRADLING, AC
影响因子：
4.6
作者：
Liu, Li;Qi, Hongying;Lin, Haifan
通讯作者：
Lin, Haifan