Screening of peptide probe binding to particulate matter with a high metal content.
Screening of peptide probe binding to particulate matter with a high metal content.
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用高金属含量筛选肽探针与颗粒物的结合。
DOI：
10.1039/c7ra13290e
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发表时间：
2018-02-02
期刊：
影响因子：
3.9
通讯作者：
Okochi, Mina
中科院分区：
文献类型：
--
作者：
Tanaka, Masayoshi;Alvin, Aw Wei Liang;Okochi, Mina
颗粒物（PM）正在成为一个日益严重的健康问题，需要开发检测方法来控制其有害影响。PM产生的活性氧（ROS）通常与金属化合物有关，因此我们的目的是筛选肽探针，以改善对具有高金属含量的PM的收集和检测。肽是推定的识别分子，由于其多功能性和易于修饰以增强其结合选择性。使用肽阵列筛选PM结合肽，并观察到根据不同斑点颜色（黄色、混合色和灰色）的不同结合行为，表明结合的PM的不同组成。与WQDFGAVRSTRS（我们以前的研究中从噬菌体展示中筛选的肽）相比，最强的结合肽被鉴定如下：NHVNTNYYPTLH（灰色）、NGYYPHSHSYHQ（混合）和HHLHWPHHHSYT（黄色），相对结合率为125%、144%和136%。电感耦合等离子体质谱（ICPMS）分析表明，结合HHLHWPHHHSYT肽点的颗粒物中Co、Ni和Zn的含量分别是未结合肽点颗粒物的12.5、15.8和7.8倍，表明该肽探针适用于高金属含量颗粒物的采集。使用肽阵列，筛选与具有高金属含量的颗粒物质结合的肽，并通过关注不同的斑点颜色（黄色、混合色和灰色）来表征。
Particulate matter (PM) is becoming an increasing health concern and there is a need to develop detection methods to keep its harmful effects in check. Generation of reactive oxygen species (ROS) by PM is often associated with metal compounds, hence our aim is to screen for a peptide probe towards improved collection and the detection of PM having a high metal content. Peptides are putative recognition molecules due to their versatility and ease of modification to enhance their binding selectivities. PM binding peptides were screened using the peptide array and different binding behaviors in terms of different spot colors (yellow, mixed and gray), indicating the different composition of bound PMs, were observed. The strongest binding peptides were identified as follows: NHVNTNYYPTLH (gray), NGYYPHSHSYHQ (mixed) and HHLHWPHHHSYT (yellow), with relative binding ratios of 125%, 144% and 136%, in comparison with WQDFGAVRSTRS, a peptide screened from a phage display in our previous study. Inductively coupled plasma mass spectrometry (ICPMS) analyses revealed that Co, Ni and Zn content in the PM bound to the HHLHWPHHHSYT peptide spot were respectively 12.5, 15.8 and 7.8 times that of the PM bound to no peptide spot, suggesting this peptide probe is applicable to collect PM with a high metal content. Using peptide array, peptides binding to particulate matter with high metal content were screened and characterized by focusing on the different spot colors (yellow, mixed and gray).
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