Tibial compression is anabolic in the adult mouse skeleton despite reduced responsiveness with aging.
Tibial compression is anabolic in the adult mouse skeleton despite reduced responsiveness with aging.
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DOI：
10.1016/j.bone.2011.05.017
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发表时间：
2011-09
期刊：
影响因子：
4.1
通讯作者：
van der Meulen, Marjolein C. H.
中科院分区：
文献类型：
--
作者：
Lynch, Maureen E.;Main, Russell P.;Xu, Qian;Schmicker, Thomas L.;Schaffler, Mitchell B.;Wright, Timothy M.;van der Meulen, Marjolein C. H.
骨干皮质骨中骨骼适应机械刺激的能力随着年龄的增长而减弱，使得成人骨形成困难。然而，年龄对松质骨适应性的影响，随着年龄的增长，组织优先丢失，没有得到很好的表征。为了建立早期绝经后妇女的模型，并确定衰老对成年小鼠骨骼松质骨适应性的影响，使用载荷参数、峰值施加载荷和峰值骨干应变幅度对成年（26周龄）骨质疏松雌性小鼠施加体内胫骨压缩，这些载荷参数先前在年轻生长（10周龄）小鼠中发现是成骨的。载荷匹配组接受与幼龄小鼠相同的峰值施加载荷（对应于胫骨内侧骨干+2100 με），应变匹配组接受与幼龄小鼠相同的峰值骨干应变（+1200 με，需要一半载荷）。使用显微计算机断层扫描和体内结构刚度测量评估了机械负荷对成年小鼠骨量和结构的影响。适应仅发生在载荷匹配组的干骺端和骨干间室中。松质骨通过骨小梁增厚增加了54%，皮质骨通过骨髓收缩和骨膜扩张增加了41%。成年小鼠能够对合成代谢刺激做出反应，并将骨量恢复到生长中小鼠的水平;然而，对于相同的施加负荷，相对于10周龄雌性小鼠的适应性反应降低。使用这种成骨加载方案，可以使用成年骨质减少小鼠模型来解决影响病理性骨丢失的其他因素。
The ability of the skeleton to adapt to mechanical stimuli diminishes with age in diaphyseal cortical bone, making bone formation difficult for adults. However, the effect of aging on adaptation in cancellous bone, tissue which is preferentially lost with age, is not well characterized. To develop a model for early post-menopausal women and determine the effect of aging on cancellous bone adaptation in the adult mouse skeleton, in vivo tibial compression was applied to adult (26 wk old) osteopenic female mice using loading parameters, peak applied load and peak diaphyseal strain magnitude, that were previously found to be osteogenic in young, growing (10 wk old) mice. A Load-Matched group received the same peak applied loads (corresponding to +2100 με at the medial diaphysis of the tibia) and a Strain-Matched group received the same peak diaphyseal strains (+1200 με, requiring half the load) as the young mice. The effects of mechanical loading on bone mass and architecture in adult mice were assessed using micro-computed tomography and in vivo structural stiffness measures. Adaptation occurred only in the Load-Matched group in both the metaphyseal and diaphyseal compartments. Cancellous bone mass increased 54% through trabecular thickening, and cortical area increased 41% through medullary contraction and periosteal expansion. Adult mice were able to respond to an anabolic stimulus and recover bone mass to levels seen in growing mice; however, the adaptive response was reduced relative to that in 10 wk old female mice for the same applied load. Using this osteogenic loading protocol, other factors affecting pathological bone loss can be addressed using an adult osteopenic mouse model.
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影响因子：
4.2
作者：
RUBIN, CT;BAIN, SD;MCLEOD, KJ
通讯作者：
MCLEOD, KJ
影响因子：
3.3
作者：
Lynch, Maureen E.;Main, Russell P.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
影响因子：
4.1
作者：
Saxon, LK;Robling, AG;Turner, CH
通讯作者：
Turner, CH
影响因子：
168.9
作者：
Heinonen, A;Kannus, P;Vuori, I
通讯作者：
Vuori, I
影响因子：
2.8
作者：
RUBIN, CT;LANYON, LE
通讯作者：
LANYON, LE