Plasma metabonomics study on Chinese medicine syndrome evolution of heart failure rats caused by LAD ligation.
Plasma metabonomics study on Chinese medicine syndrome evolution of heart failure rats caused by LAD ligation.
复制标题
LAD结扎心力衰竭大鼠中医证候演变的血浆代谢组学研究
DOI：
10.1186/1472-6882-14-232
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发表时间：
2014-07-09
影响因子：
--
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Qiu Q;Li C;Wang Y;Xiao C;Li Y;Lin Y;Wang W
研究背景中医证候在许多疾病模型中尚未得到明确的表征和验证，中医证候的概念也存在混淆和争议。代谢组学已被应用于临床和非临床研究中的中医证候评价和分类。在这项研究中，我们的目的是探讨心肌梗死导致的心力衰竭中医证候的演变，并确认在证病结合动物模型的血浆代谢组学郑分类的可行性。方法心力衰竭（HF）模型通过结扎左冠状动脉前降支（LAD）在SD大鼠。将大鼠分为两组：心衰模型组（结扎左前降支）和假手术组。结果缺血性心力衰竭气虚血瘀证的稳定时间窗为7 ~ 28 d，缺血性心力衰竭气虚血瘀证的稳定时间窗为21 d，气虚血瘀证的稳定时间窗为28 d。术后1 ~ 4d和45 ~ 60 d出现气虚证。结果显示，LAD术后4和21天有5种血浆代谢物变化趋势相同，21和45天有7种，4和45天有2种。所有3个时间点的代谢产物均未显示相同变化。在术后第21天（QDBS综合征时间点），4种血浆代谢物的变化趋势与我们以前对血瘀证患者的研究结果一致。血浆代谢产物可为中医证候动物模型的评价提供依据。
BackgroundChinese medicine syndromes (Zheng) in many disease models are not clearly characterized or validated, and the concepts of Chinese medicine syndromes are confounding and controversial. Metabonomics has been applied to the evaluation and classification of the Chinese medicine syndromes both in clinical and nonclinical studies. In this study, we aim to investigate the evolution of the Chinese medicine syndrome in myocardial infarction induced heart failure and to confirm the feasibility of the Zheng classification by plasma metabonomics in a syndrome and disease combination animal model.MethodsThe heart failure (HF) model was induced by ligation of the left anterior descending coronary artery (LAD) in Sprague–Dawley rats. The rats were divided into the following two groups: the HF model group (LAD ligation) and the sham operated group. GC-MS was used with pattern recognition technology and principal component analysis (PCA) to analyze the plasma samples at 4, 21 and 45 day after operation.ResultsIt was determined that the period from 7 to 28 days was the stable time window of ischemic heart failure with qi deficiency and blood stasis syndrome (QDBS), and the qi deficiency syndrome occurred at 1 to 4 days and 45 to 60 days after operation. The results exhibited 5 plasma metabolite changes in the same trend at 4 and 21 day after the LAD operation, 7 at 21 and 45 day, and 2 at 4 and 45 day. No metabolite showed the same change at all of the 3 time points. At day 21 (the QDBS syndrome time point) after operation, 4 plasma metabolites showed the same trends with the results of our previous study on patients with the blood stasis syndrome.ConclusionsThe syndrome diagnosis is reliable in the HF rat model in this study. Plasma metabolites can provide a basis for the evaluation of Chinese medicine syndrome animal models.
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影响因子：
5.4
作者：
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