Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen.
Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen.
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DOI：
10.1038/ncomms4591
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发表时间：
2014-04-07
影响因子：
16.6
通讯作者：
Ochiya, Takahiro
中科院分区：
文献类型：
--
作者：
Yoshioka, Yusuke;Kosaka, Nobuyoshi;Konishi, Yuki;Ohta, Hideki;Okamoto, Hiroyuki;Sonoda, Hikaru;Nonaka, Ryoji;Yamamoto, Hirofumi;Ishii, Hideshi;Mori, Masaki;Furuta, Koh;Nakajima, Takeshi;Hayashi, Hiroshi;Sugisaki, Hajime;Higashimoto, Hiroko;Kato, Takashi;Takeshita, Fumitaka;Ochiya, Takahiro
癌细胞分泌小的膜性细胞外囊泡（EV）进入其微环境和循环。尽管它们作为癌症生物标志物的潜力很有希望，但临床样品中EV的鉴定和定量仍然具有挑战性。在这里，我们描述了一种灵敏和快速的分析技术，用于直接从结直肠癌患者的血液样本中分析循环EV。EV被两种类型的抗体捕获，并被光敏珠检测到，这使我们能够在没有纯化步骤的情况下检测癌症衍生的EV。我们还表明，循环EV可用于使用嵌入癌症相关EV中的抗原CD147检测结直肠癌。这项工作描述了一种新的液体活检技术，灵敏地检测疾病特异性循环EV，并从诊断和治疗的角度提供了转化医学的观点。 细胞外囊泡（EV）作为癌症生物标志物的潜力是巨大的。在这里，Yoshioka等人描述了一种直接从结直肠癌患者的血液样本中分析EV的灵敏技术，突出了具有癌症检测可能性的液体活检技术。
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy. The potential of extracellular vesicles (EVs) as cancer biomarkers is substantial. Here, Yoshioka et al. describe a sensitive technique to analyse EVs directly from blood samples of patients with colorectal cancer, highlighting a liquid biopsy technique with cancer-detection possibilities.
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影响因子：
11.2
作者：
Bobrie, Angelique;Krumeich, Sophie;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
6.4
作者：
Li, Yu;Xu, Jing;Chen, Zhi-Nan
通讯作者：
Chen, Zhi-Nan
影响因子：
8.8
作者：
Boye, K.;Nesland, J. M.;Sandstad, B.;Haugen, M. Haugland;Mlandsmo, G. M.;Flatmark, K.
通讯作者：
Flatmark, K.
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
3.4
作者：
Mathivanan, Suresh;Simpson, Richard J.
通讯作者：
Simpson, Richard J.