Prolonged cold ischemia does not trigger lethal rejection or accelerate the acute rejection in two allogeneic rat liver transplantation models.
Prolonged cold ischemia does not trigger lethal rejection or accelerate the acute rejection in two allogeneic rat liver transplantation models.
复制标题
在两种同种异体大鼠肝移植模型中，长时间冷缺血不会引发致死性排斥反应或加速急性排斥反应
DOI：
10.1016/j.jss.2011.03.078
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Dirsch O
中科院分区：
文献类型：
--
作者：
Dahmen U;Huang H;Dirsch O
长期冷缺血（CI）可能会诱导BN-Lew和ACI-Lew肝移植（LT）模型中的致死性肝移植排斥反应和/或加速致死性排斥反应的过程，这已成功地挑战了肝脏大小reduction.MATERIALS和METHODS受体的存活率和体重发展后，接受移植物在盐水中长期CI进行了评估。根据组织学和门静脉浸润的大小评估排斥反应的严重程度。观察肝组织中CD 25、CD 28、CD 38、颗粒酶B和穿孔素的表达，进一步判断同种异体免疫反应的严重程度。然而，同种异体免疫反应的增强并不能逆转BN-Lew肝移植的自发接受到致死性排斥反应。8小时CI导致100%的4周存活率和移植物接受性，并且不影响术后体重恢复。9 h CI导致2/6只大鼠在48 h内因移植物原发性无功能（PNF）而死亡。10 h CI在96 h内引起所有大鼠的致死性PNF。存活受体的组织学检查显示，由于炎性浸润，门脉扩张。在死于PNF的大鼠中，观察到广泛的小叶中心坏死，但没有排斥反应的迹象。在ACI-Lew全尺寸LT模型中，8 h CI并不加速致死性排斥反应的过程。结论CI延长与同种异体免疫反应的离散增强有关，但在BN-LEW自发性肝移植接受模型中并不触发致死性排斥反应，在ACI-Lew排斥反应模型中并不加速急性排斥反应。
BACKGROUNDProlonged cold ischemia (CI) might induce lethal liver graft rejection and/or accelerate the course of lethal rejection in BN-Lew and ACI-Lew liver transplantation (LT) models, which have been successfully challenged by liver-size reduction.MATERIALS AND METHODSSurvival rate of recipients and body weight development after subjecting the grafts to prolonged CI in saline were assessed. Severity of rejection was assessed based on histology and the size of portal infiltration. Hepatic expression of CD25, CD28, CD38, granzyme B, and perforin were assessed to further judge the severity of allogeneic immune response.RESULTSAn enhancement of allogeneic immune response after prolonged CI was observed, indicated by up-regulated mRNA expression of CD25, CD28, CD38, granzyme B, and perforin. However, the enhancement of allogeneic immune response did not reverse the BN-Lew spontaneous liver graft acceptance to lethal rejection. Eight-hour CI led to 100% 4 wk survival rate and graft acceptance and did not impair postoperative body weight recovery. Nine-hour CI caused the death of 2/6 rats due to primary non-function (PNF) of the graft within 48 h. Ten-hour CI caused lethal PNF in all rats within 96 h. Histology of surviving recipients revealed an expansion of the portal tract due to an inflammatory infiltrate. In rats dying from PNF, extended centrilobular necrosis was observed, but no sign of rejection. In the ACI-Lew full-size LT model, 8 h CI did not accelerate the course of lethal rejection.CONCLUSIONProlonged CI was associated with an discrete enhancement of allogeneic immune response, but did not trigger lethal rejection in BN-LEW spontaneous liver graft acceptance model and did not accelerate acute rejection in ACI-Lew rejection model.
登录
查看更多内容
扩大肝切除大鼠模型中内参基因的定量评估和选择。
DOI：
10.1016/s1726-4901(08)70161-8
发表时间：
2009
期刊：
Journal of biomolecular techniques : JBT
影响因子：
--
作者：
W. Xing;M. Deng;Jinyan Zhang;Hai Huang;O. Dirsch;U. Dahmen
通讯作者：
U. Dahmen
反义 B7.1 的基因转移可减轻大鼠同种异体肝脏移植物的急性排斥反应
影响因子：
1.9
作者：
Y. Mi;Rui Li;Kesen Xu;Hongchi Jiang;Xueying Sun
通讯作者：
Xueying Sun
丁丙诺啡对 ACI 和 BN 大鼠近交系行为的影响
DOI：
--
发表时间：
2008
期刊：
Laboratory Animals. Journal of the Laboratory Animal Science Association
影响因子：
--
作者：
H. Avsaroglu;R. Sommer;L. Hellebrekers;L. van Zutphen;H. V. van Lith
通讯作者：
H. V. van Lith
小尺寸肝移植后诱导排斥反应：尺寸很重要
影响因子：
1.9
作者：
O. Dirsch;Jun Li;Qing He;Yuan Ji;Yanli Gu;U. Dahmen
通讯作者：
U. Dahmen
肝移植物可以保存过夜。
DOI：
--
发表时间：
1996
期刊：
Liver Transplantation and Surgery
影响因子：
--
作者：
J. Vix;P. Compagnon;J. Beller;D. Jaeck;P. Wolf;K. Boudjema
通讯作者：
K. Boudjema